GDP-fucose protein O-fucosyltransferase 2 (POFUT2) is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues in Thrombospondin repeats. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and...
The wbsJ gene from Escherichia coli O128:B12 encodes an al,2-fucosyltransferase responsible for adding a fucose onto the galactose residue of the 0-antigen repeating unit via an α1,2 linkage. The wbsJ gene was overexpressed in E. coli BL21 (DE3) as a fusion protein with glutathione S-tra...