因此,脂质、聚合物、树突分子和天然膜基的mRNA药物递送系统已经被开发出来,用于将mRNA递送到靶细胞。为了将其中一些系统应用于临床,已经作出了重大努力和投资。具体而言,系统给药的脂质纳米颗粒(LNPs)已将mRNA传递到肝脏,而肌内给药的LNPs已将mRNA传递到免疫细胞,以预防2019年的冠状病毒病。然而,在非肝组织,如脾脏...
Polychlorinated biphenyls (PCBs) have been shown to also reduce hepatic Fgf21 mRNA19. Since TCS and other environmental toxicants can reduce FGF21 expression, NAFLD caused by environmental toxicants can be more aggressive than the development of NAFLD caused by HFD. However, the exact mechanism ...
In the meantime, the intracellular luciferase was introduced by lipid nanoparticle (LNP)-mediated fLuc mRNA delivery, rendering it suitable for bioluminescence (BL) imaging in vitro and in vivo. Based on this luciferase鈥搇uciferin system, the endogenous H2S could be sensitively and selectively ...
The TLR mRNA production in the gut mucosa of mice with colonic visceral hypersensitivity was studied and significant increases were seen[140]. Similar results were found in humans[141]. TLRs are fundamental in T-cell differentiation and activation, particularly for Th17 and Treg cells[142]. In ...
3 mRNA was tested by semi-quantitative RT-PCR.Results:mRNA expressions of SOSC-3 in liver of the control group,TCM groups and western medicine group were significantly lower than those of model group(P0.01).Conclusion:DSC can reduce the mRNA expressions of SOCS-3 of liver in rats with ...
(AAV) delivery. Flox and F4MKO mice were first modeled with NASH. After 24-week NASH modeling, AAV-FSTL1, tagged with flag, was injected into the GAS. After 4 weeks of the AAV injection, the mRNA expression of FSTL1 in GAS was increased significantly in the AAV-FSTL1-treated group (...
This miRNA may then, via the miRNP, affect gene expression through several different mechanisms depending on the miRNA-mRNA pair in question and the cellular context. Binding of the miRNP to target mRNA sequences can lead to mRNA degradation (A) or repression of mRNA translation into protein ...
by intravenous delivery. Here we first compare engineered coagulation factor VIII transgenes and show that codon-usage optimization improved expression 10-20-fold in hemophilia A mice and that inclusion of an unstructured XTEN peptide, known to increase the half-life of the payload protein, provided...
In addition, another study found that miR-223 in bone marrow mesenchymal stem cell-derived exosomes (BmMSC-Exo) also inhibited NLRP3 activation by binding to the NLRP3 3′-untranslated region (3′-UTR), thus leading to NLRP3 mRNA degradation, which suppressed liver inflammation and cell death...
Insulin: Ultra-sensitive mouse insulin ELISA kit (Crystal Chem, # 90090) was used to determine insulin, Triglycerides were determined using Cardiochek Lipid panel test strips. qRT-PCR: mRNA was extracted from adipose tissue of high-fat fed mice using the RNeasy Mini kit as per the manufacturer...