An NMR-based alternative to traditional X-ray crystallography and NMR methods for structure-based drug design is described that enables the structure determination of ligands complexed to virtually any biomolecular target regardless of size, composition, or oligomeric state. The method utilizes saturation...
Here we provide an introduction to NMR-based approaches for studying protein dynamics, highlighting eight distinct methods with recent examples, contextualized within a common experimental and analytical framework. The selected methods are (1) Real-time NMR, (2) Exchange spectroscopy, (3) Lineshape ...
Additionally, considerations and methods for data analysis, including multivariate statistics, univariate statistics, and data interpretation are included. The process is illustrated by examining the metabolic effects of chemical treatment of Arabidopsis with Sortin 1, also known as vacuolar protein sort ...
The focus of this report concerns the use of nuclear magnetic resonance (NMR) spectroscopy for metabolic analyses and, in particular, its use in toxicology for examining the metabolic profile of biofluids. Examples from the literature will demonstrate how 1H NMR and pattern recognition methods are ...
An Efficient High-Throughput Resonance Assignment Procedure for Structural Genomics and Protein Folding Research by NMR Sequence specific resonance assignment is the primary requirement for all investigations of proteins by NMR methods. In the present postgenomic era where s... Neel,S.,Bhavesh,... -...
S1. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) 4. Methods 4.1. Protein expression and purification E. coli EI and uniformly 15N-labelled EIC, EIN, and HPr were expressed and purified as previously ...
In recent years a lot of effort has been made to identify the highest priority needs in order to improve the diagnostic procedures for TB1,19. The need to develop accurate and more accessible diagnostic methods in primary healthcare centres has meant an intensification of the search for biomarke...
Methods Endo-therapy device The device used for the endo-therapy application is depicted in Fig.7. It contains an extremely thin and arrow-shaped metal tip designed for precise delivery of the active ingredients into the plant vascular system (Fig.7a). The reduced tip dimensions (8.5 mm length...
CRP C-reactive protein HBI Harvey-Bradshaw Index HMDB Human Metabolome Database IBD Inflammatory Bowel Disease IBDQ Inflammatory Bowel Disease Questionnaire IL Interleukin ITT intention-to-treat NMR Nuclear magnetic resonance OPLS DA Orthogonal Projections to Latent Structures Discriminant Analysis PCA pri...
Materials and Methods Chemicals and reagents. Dulbecco's modified Eagle medium (DMEM) was purchased from Gibco (Carlsbad, CA). Fetal bovine serum (FBS) was purchased from Biowest. ViraPower adenovirus expression sys- tem and Gateway system were obtained from Invitrogen (Carlsbad, CA). Cell-...