Robo1-CAR-NK92 cells).Conclusions Compared to parental NK92 cells,Robo1CAR-NK92 cells exhibited more potent targeted killing against glioma and neuroblastoma cells.Collectively,treatment of IL-15 and dexamethasone was the optimized protocol for culture of Robo1 CAR NK cells during our experim...
Following this step, the infection protocol was repeated. Starting from day 1 after the second infection, transduced NK92 cells were purified using a FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) based on expression of a GFP marker on the cell surface encoded by the vector...