1. 使用方便,该NK Cell培养kit包含了几乎所有必须的组分,除白介素2(IL-2)之外无需其他培养基,抗体或细胞因子。整个使用流程方便快捷。具体使用流程如下: Figure 1 protocol of human NK cell expansion 2. 只需要30ml外周血即可开始实验。 3. 效应细胞比例高。经该Kit培养外周血单核细胞14天,效应细胞(NK和NKT...
[4] Juliane W , Viktoria P , Anja W ,et al.A Two-Phase Expansion Protocol Combining Interleukin (IL)-15 and IL-21 Improves Natural Killer Cell Proliferation and Cytotoxicity against Rhabdomyosarcoma[J].Frontiers in Immunology, 2017, 8:676-. [5] Markus G , Juliane ...
Therefore, coculture systems of irradiated feeder cells and NK cells in media containing IL-2 and IL-15 have been developed to generate large numbers of NK cells, although NK cell expansion protocol using anti-CD3 antibody (OKT-3) without feeder cells has also been developed. Commonly used ...
▲ This NK cell activation and expansion protocol is optimized for NK cells that have been purified using the NK Cell Isolation Kit, using one loaded Anti-Biotin Microbead Particle per two NK cells (bead-to-cell ratio 1:2). ▲ Volumes for activation given below are for 106 purified NK cel...
Methods of expanding a natural killer (NK) cell fraction for transplantation into a subject are provided, and particularly, methods for providing transplantable NK cell fractions and protocols for their use, which can be employed for applications in cell transplants and infusions for treatment of ...
In summary, the large amount of cytotoxic NK cells generated by this ex-vivo expansion protocol provides the numbers of NK cells that will probably be required to be effective in the case of a large tumor burden. The ability of the expanded cells to mediate ADCC offers the possibility that ...
This application note describes the protocol for rapid expansion of NK cells using the Corning NK Expansion kit, as well as tests that validate the use of the kit. The results support the use of the Corning NK Expansion kit by researchers who need high expan- sion capacity, high viability,...
In summary, the large amount of cytotoxic NK cells generated by this ex-vivo expansion protocol provides the numbers of NK cells that will probably be required to be effective in the case of a large tumor burden. The ability of the expanded cells to mediate ADCC offers the possibility that ...
In summary, the large amount of cytotoxic NK cells generated by this ex-vivo expansion protocol provides the numbers of NK cells that will probably be required to be effective in the case of a large tumor burden. The ability of the expanded cells to mediate ADCC offers the possibility that ...