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NF-κB TWO-Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor cell viability alongside NF-κB (nuclear factor Kappa B) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element lo
response elements, inducing transcription of the luciferase reporter gene. Format Each vial contains 2 X 10^6 cells in 1 ml of 10% DMSO. Mycoplasma Testing The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of ...
Cell Microbiol. 9(3):694-707. Figures Validation of MDA-5 knockout by Western blot (Wes™) IRF INDUCTION (Lucia luciferase reporter) NF-κB INDUCTION (SEAP reporter) Back to the top Specifications Antibiotic resistance: Zeocin®, blasticidin Growth Medium: DMEM, 4.5 g/l glucose, 2...
Importantly, NPI-1387 reduced TNF-A-induced NF-KB activation in a HEK293 NF-KB/luciferase reporter cell line. Therefore additional studies were initiated to define the biological activities in MM. Treatment of MM cells lines (MM.1S, MM.1R, OCI-My5, OPM1, Dox-40) with NPI-1347 for 48...
AnalysisofSL-0013NFκBreporteractivityinresponsetoTNFαtreatment.TheMCF-7cellswereseededona96-wellplateforovernightincompletegrowthmedia. Thecellsthenweretreatedwithorwithout20ng/mlTNFαingrowthmediawith0.1%FBSfor16hours. MCF-7-NFkBLuciferaseReporterCellLineexhibitsTNFα-dependentincreaseinluciferaseactivitywhen...
NF-κB luciferase reporter plasimd 荧光素酶报告基因(报告基因质粒) Genomeditech 1μg 800 在线询价 翌圣生物科技(上海)股份有限公司 类型: 生产厂家 联 系 人: 曹女士 电话: 400-6111-883 传真: 86-21-34615995 手机: 联系我时,请说明在化工仪器网上看到的,谢谢。
cGAS knockout NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes THP1-Dual KO-cGAS pathway THP1-Dual™ KO-cGAS cells were generated from THP1-Dual™ cells by stable knockout of the cGAS gene. They derive from human THP-1 monocytes, a cell line often used to study DNA sensing...
5、.cornABSTRACT:ObjectiveToelvaluatetheeffectoflowsalt(LS)ontheexpressionofcyclooxygenase-2(COX-2)andtheactivityofnuclearfactorkappaB(NFKs)andactivatorprotein一1(AP一1)inthemousemaculadensaderived(MMDDI)cellline.MethodsMMDDIcellsweretransfectedwithluciferasereporterplasmidcontainingAP一1orNFKB.Luciferase 6、...
Similar results of p-p65 elevation were found in a human colon epithelial cell line compared with the REGg knockdown controls (Supplementary Fig. 2B). To validate REGg-dependent regulation of p65 signalling, NFkB luciferase reporter activity was measured upon REGg overexpression or depletion in ...