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This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-ĸB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response...
MCF-7-NFkBLuciferaseReporterCellLineexhibitsTNFα-dependentincreaseinluciferaseactivitywhencomparedtountreatedcells. Signosis基因功能分析,靶标发现和确认,分析开发以及化合物筛选通常需要基于细胞的分析。经过工程改造以通过转基因整合到宿主基因组中表达目的基因的稳定细胞系提供了进行此类分析的有效方法。然而,由于建立...
5、.cornABSTRACT:ObjectiveToelvaluatetheeffectoflowsalt(LS)ontheexpressionofcyclooxygenase-2(COX-2)andtheactivityofnuclearfactorkappaB(NFKs)andactivatorprotein一1(AP一1)inthemousemaculadensaderived(MMDDI)cellline.MethodsMMDDIcellsweretransfectedwithluciferasereporterplasmidcontainingAP一1orNFKB.Luciferase 6、...
Importantly, NPI-1387 reduced TNF-A-induced NF-KB activation in a HEK293 NF-KB/luciferase reporter cell line. Therefore additional studies were initiated to define the biological activities in MM. Treatment of MM cells lines (MM.1S, MM.1R, OCI-My5, OPM1, Dox-40) with NPI-1347 for 48...
(MMDDI)cellline.MethodsMMDDIcellsweretransfectedwithluciferasereporterplasmidcontainingAP一1orNF—KB.Luciferasereporterassaywasusedtoevalu~etheeffectofnormalsalt(NS)andlOWsalt(LS)ontheactivitiesofNF.KBandAP.1.ThechangesofCOX-2expressionwereexam.inedbyRT—PCR.Theexpressionofp-p38MAPK,p-p44/42,c—Jun,...
Cell extracts were prepared and tested for luciferase activity. Results were normalized for transfection efficiencies with respect to beta galactosidase activity. Result represents the mean ±s.d. of three independent experiments. (c) Interaction of c-Rel with p50 and I-kBe in A549 cells. Cells ...
fFunction examined by luciferase reporter assay. gFunction examined by enzyme-linked immunosorbent assay. hFunction examined by reverse transcriptase PCR. iFunction examined by electrophoretic mobility shift assay. (IL1RN T4C (rs4251961)), decrease IL6 expression (IL6 –6331T4C (rs10499563)) and ...
regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when usingQUANTI-Blue™ Solution, a SEAP detection reagent, andQUANTI‑Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection ...
Nfkb1–1750-Luc, containing −1735/37 bp of the murine nfkb1 promoter linked to a luciferase reporter, was cotransfected with empty CMV vector or with CMV expression plasmids encoding C/EBPα, C/EBPαLZ, C/EBPαBR3, or NF-κB p50, as well as CMV–β-Gal as an internal control....