In this study, we propose a strategy that integrates ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) and bioactive (NF-κB inhibitor) luciferase reporter assay systems for the rapid determination of various anti-inflammatory compounds of TCM ...
We analyzed promoter activities of these regions with a luciferase-based reporter assay system. A 64-bp region was identified to contain the transcription initiation site and a minimum promoter sequence for transcriptional activation of the USP24 gene expression. Expression of USP24 is controlled by ...
The dual luciferase reporter system was performed to analyze the regulatory relationship between E2F1 and MMPs. Results E2F1 is an independent and adverse prognosis factor that is highly expressed in SCLC in a Chinese Han population. Knockdown of E2F1 by specific siRNA resulted in the downregulation ...
1A, NLK strongly down-regulated the luciferase activity of the NF-κB reporter. Moreover, overexpression of NLK inhibited the TNFα-triggered activation of NF-κB in a dose-dependent manner (Fig. 1B). In addition, quantitative RT-PCR (qRT-PCR) analyses indicated that NLK suppressed the ...
www.nature.com/scientificreports OPEN Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions received: 11 October 2016 accepted: 13 February 2017 Published: 20 March 2017 Chen Zhang1, Hui Jiang2, Pin Wang3, Heng Liu3 & Xiulian Sun4 ...
Activation of NF-κB was monitored by expression of luciferase from cells transfected with an NF-κB dependent luciferase reporter construct. 70Z/3 murine pre-B cells and U937 human monocytes failed to produce luciferase in response to 10 7 pneumococci or 10 μ g cell wall; strong responses ...
An ability to activate NF-κB refers to an ability to increase the reporter activity (luciferase activity) relative to the control experiment (cells into which only a null vector was introduced). Increase in reporter activity is preferably by a factor of 1.5 or more, more preferably by a ...
Results of NF-κB response element luciferase reporter assay confirmed that MB and MZ activated NF-κB. The NF-κB inhibitor (SN50) was also shown to alleviate cytotoxicity induced by co-treatment of MB or MZ and MPP+. This study demonstrates that activation of NF-κB is responsible for ...
B response gene expression was evaluated using a luciferase reporter. Cell viability was assessed by Trypan blue staining or ATP luminescent assay while apoptosis was evaluated by caspase-3 activity. Results CCN2 induced I??B?? phosphorylation and degradation as well as nuclear accumulation of NF-...
In this study, we used an NF-kappa B luciferase reporter gene system. Out of more than 800 compounds screened, four NF-kappa B agonists were identified with strong activity at nontoxic concentrations. Subsequently, the adjuvant effect was verified on mouse bone marrow-derived dendritic cells (...