Mann DA (2002) The NFkappaB luciferase mouse: a new tool for real time measurement of NFkappaB activation in the whole animal. Gut 51, 769-770.Mann DA. The NFkappaB luciferase mouse: a new tool for real time measurement of NFkappaB activation in...
We analyzed promoter activities of these regions with a luciferase-based reporter assay system. A 64-bp region was identified to contain the transcription initiation site and a minimum promoter sequence for transcriptional activation of the USP24 gene expression. Expression of USP24 is controlled by ...
2E). Luciferase reporter assay showed that the activity of NF-κB was higher in the NLK−/− cells in comparison to the parental HCT116 cells regardless of the TNFα stimulation (Fig. 3A). Consistent with the notion that NLK negatively regulates NF-κB signaling in a kinase-independent ...
ChIP-to-sequence and promoter-based luciferase analysis demonstrated that E2F1 directly controlled MMP-16 expression via an E2F1 binding motif in the promoter. Although one E2F1 binding site was predicted in the MMP-9 promoter, luciferase analysis indicated that this binding site was not functionally...
Rather, stimulation of MMNK-1 cells with LPS, but not TGFβ1, caused IκBα degradation and activation of a NF-κB-responsive luciferase construct. Expression of an IκBα super-repressor or dominant negative IKK2 inhibited activation of the NF-κB-responsive luciferase construct and attenuated...
www.nature.com/scientificreports OPEN Transcription factor NF-kappa B represses ANT1 transcription and leads to mitochondrial dysfunctions received: 11 October 2016 accepted: 13 February 2017 Published: 20 March 2017 Chen Zhang1, Hui Jiang2, Pin Wang3, Heng Liu3 & Xiulian Sun4 ...
In contrast, THP-1 human monocytes showed strong luciferase production with all three stimuli: LPS, intact pneumococci and cell wall. The response was time and dose dependent. Cell wall activity was retained despite alteration of the choline of the teichoic acid or protease treatment suggesting the...
After 24 or 48 hours of culture, luciferase activity was measured, and the plasmid with significantly increased luciferase activity compared to that of a control experiment (vector pME 18S-FL3 is introduced into a cell in place of a full-length cDNA) was selected (the selected plasmid showed ...
ACT001 effectively suppressed the growth of U-118 MG tumors in BALB/c nude mice and GL-261-luciferase tumors in C57BL/6 J mice. Finally, ACT001 downregulated the expression of p-p65, MnSOD, cyclin B1, CDC2, and Ki67 in U-118 MG tumor tissues. Patients with activated NF-kappa B ...
The reporter construct showed that the NF-kappa B BSs could activate the luciferase expression in cells in a NF-kappa B-specific manner. The quantitative PCR and Western blot detections demonstrated that NF-kappa B could regulate the expressions of IDH1, IDH3A, and ACO2 genes at both mRNA ...