[0030]用Promega公司的Dual-LuciferaseReporterAssaySystem检测样品 Luciferase活性。按上述转染方法转染细胞。转染48h后,吸去旧培养基,用PBS清洗两次, 每孔细胞加入100y1的PassiveLysisBuffer(PLB),室温轻微振摇15min。收集细胞裂解 液于I. 5mlEppendorf管中;向Eppendorf管中加入 100U1 的LuciferaseAssayReagent II(LARII...
A NF-kB luciferase reporter gene stimulated by IL-1b was inhibited in a dose-dependent manner by ARTD10 in HeLa cells (Fig. 2a). Mutation of either the active centre in ARTD10-G888W, which is devoid of catalytic activity, or the UIMs in ARTD10-DUIM was not sufficient to ...
7e). To further verify this hypothesis, we used a dual-luciferase reporter assay to test the effect of ectopic expression of NF-κB/p65 on the LINC01410 promoter activity. Ectopic expression of NK-κB/p65 enhanced the transcription of firefly luciferase driven by the wild-type LINC01410 ...
Last, luciferase reporter assay was performed to study the transcriptional regulation of EV on the NF‐kB and P38 pathway. The results of our study suggested that treatment with 0, 1, 2 or 4mmol/L ketamine altered the morphology and secretion capacity of extracellular vesicles. As the ...
NF-κB TWO-Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor cell viability alongside NF-κB (nuclear factor Kappa B) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element lo
a The luciferase reporter assay using a STAT3-Luc (a) reporter plasmids. H. pylori infection of AGS-pcDNA cells significantly increased the luciferase activity, which was reduced after treatment with BAY (10 µM) or transfection with Iκ-B super-repressor plasmid The bar graphs ...
5、.cornABSTRACT:ObjectiveToelvaluatetheeffectoflowsalt(LS)ontheexpressionofcyclooxygenase-2(COX-2)andtheactivityofnuclearfactorkappaB(NFKs)andactivatorprotein一1(AP一1)inthemousemaculadensaderived(MMDDI)cellline.MethodsMMDDIcellsweretransfectedwithluciferasereporterplasmidcontainingAP一1orNFKB.Luciferase 6、...
(A) mel-14PA melanoma cells stably expressing the HTR control vector or a vector expressing miR-377 were co-transfected with NF-κB luciferase vector and renilla vector as an internal control. The resulting cell extracts were analyzed by luciferase reporter assay and val...
reportergene wastransfectedintoHL一60.MCF一7and Raji cellsandthe response ofthesecellstothetwo types ofTNF一Ⅱ was then studied.These results showedthat S—TNF—a could significantly increasetheluciferase activity ofHL一60andMCF一7cells buthadnoeffecton ...
(not shown). Nfkb1–1750-Luc, containing −1735/37 bp of the murinenfkb1promoter linked to a luciferase reporter, was cotransfected with empty CMV vector or with CMV expression plasmids encoding C/EBPα, C/EBPαLZ, C/EBPαBR3, or NF-κB p50, as well as CMV–β-Gal as an ...