NF-kB p65 本人自费科研博士,课题更改后有一支全新闲置的NF-kB p65抗体(CST 8242T)20ul,货真价实,现低价转让,价格低于官网4折,仅需800元一支。更新后还可开发票。抗体信息: 品牌:Cell Signaling Technology 类型:Rabbit mAb 靶标:NF-kappaB p65 规格:20ul 编号:8242T 存储条件:-20℃产品特点: 刮涂层扫二...
To detect specific protein expression by western blot, antibodies against P65 (cat. #8242), IκBα (cat. #4812) and α-tubulin (cat. #2144) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Lamin B (cat. #sc-6217) were pur- chased from Santa Cruz ...
Cell Signaling), NF-κB p65 (8242S, clone D14E12, dilution 1:2,000, Cell Signaling), phosphorylated CREB (pCREB, S133; sc-101663, dilution 1:1,000, Santa Cruz Biotechnology), pAkt (S473; 4060S, clone D9E, dilution 1:1,000, Cell Signaling), Lamin B (sc-6216, clone C-20, ...
The membrane was blocked with 5% non-fat milk at 4 °C overnight, and then incubated with anti-LPTS (#H00054984-K, Abnova), anti-p65 (#8242, Cell signaling) and anti-β- actin (#3700, Cell signaling) for 10 h at 4 °C. The mem- brane was rinsed 3 times with PBS containing ...
使用CellSignalingTechnology公司的信号通路蛋白抗体IkBa (4814S)、p-IkBaSer32(2859S)、p65(8242S)、p-p65Ser536(3033S)。AlexaFluor488 (AF488)、AlexaFluor594(AF594)荧光抗体购自美国Invitrogen公司。DAPI细胞核染料购买 于碧云天。 细胞培养试剂DMEM培养基、胎牛血清、1%青霉素-链霉素及胰蛋白酶购买于Gbico公司...
After completing total protein quantification using the BCA Protein Assay Kit (Beyotime Biotechnology), equal amounts of total protein in each group were assayed and probed with antibodies against phospho-NF-κB p65 (1:1000, CST, 8242), NF-κB p65 (1:1000, CST, 3033), and GAPDH (1:1000...
使用在牛血清白蛋白中的2%酪蛋白将nf-κb(clone 8242s,cell signaling technology,danvers,ma)和icam-1(clone af796,r&d systems)的一抗稀释至适当浓度,加到载玻片,并且在4℃下隔夜孵育。冲洗后,在22℃下施加二抗(dako,carpinteria,ca)1h。将二氨基联苯胺加入2min,并且使用苏木精复染载玻片。以400...
Normal Rabbit IgG Cell Signaling 2729, RRID: AB_1031062 Rabbit anti-p50 Cell Signaling 13586, RRID: AB_2665516 Rabbit anti-p65 Cell Signaling 8242, RRID: AB_10859369 Rabbit anti-SOCS1 Cell Signaling 3950, RRID: AB_2192983 Hamster anti-CD11c N418 (BV711) BioLegend 117349, RRID: AB_2563905...
Rabbit polyclonal antibodies against p65 (8242P), Procaspase-9 (9502S), Procaspase-3 (9664P), Procaspase-7 (9492P), Cytochrome c (4272S), PARP (9542P), RIP1 (3493), β -actin (4967S) and anti-rabbit HRP linked secondary antibody (7074P2) were purchased from Cell Signaling ...
There has been reported that LPS can induce translocation of NF-κB/p65 from the cytoplasm to the nucleus23. And the nucleus translocation of NF-κB can regulate the release of large amounts of inflammatory mediators such as TNF-α, IL-6, IL-1β, NO, and iNOS24. As shown in Fig. 3A...