IL-1b (100 ng per mouse; in tumor; twice a week; for 2 weeks) significantly reversed the growth of subcutaneous INHBB-knockdown MGC-803 xenografts (Fig. 8F-G, S10E). Also, JSH-23 (1 mg/kg; orally administered; daily; for 2 weeks) significantly suppressed the growth of subcutaneous ...
The potential downstream genes were further analyzed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) analyses. For experimental models, primary microglia and neurons were extracted from the cortical tissues of mouse brains. An in vitro...
8,21. The importance of the aspartate aminotransferases GOT1 and GOT2 in the generation of Glu and Asp from α-KG and OAA, respectively, prompted us to study whether their inhibition may suppress cell proliferation as readily as the inhibition of GLS. As shown above, both Asp and α-KG ...
Next, DAVID Bioinformatics Resources25,26 (The Database for Annotation, Visualization and Integrated Discovery, http://david. abcc.ncifcrf.gov) were used to perform Gene Ontology (GO)-based functional enrichment and KEGG pathway enrichment analyses. For immune-related gene analysis, hierarchical ...
The potential downstream genes were further analyzed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) analyses. For experimental models, primary microglia and neurons were extracted from the cortical tissues of mouse brains. An in vitro...
(E) Altered genes analysis in top categories depending on KEGG from NC-EXO vs. OE-EXO (fold change ≥ ± 2, P < 0.05) Full size image Fig. 5 OE-EXO activated the NF-kB signaling pathway and induced PANoptosis in OS cells. (A–C) Immunoblotting: Pyroptosis\Apoptosis\...
Genes related to the NF-κB signaling pathway, such as NFKB1, BAFF, and BAFFR, were up-regulated. Genes related to myelogenesis, such as PU.1, EGR1, and GFI1 were up-regulated. The rich factor plot of KEGG pathway enrichment analysis of RNA-seq results for signaling pathway also ...
To target block the NF-kB signaling pathway in tendon disease, IKKβ has been genetically deleted in mice tendon fibroblasts.12 Conditional IKKβ knockout partially protected patients from overuse-induced tendinopathy, such as that caused by treadmill overuse. Additionally, both genetic deletion and ...
(Fig.2c). Consistently, the majority of calcium genes identified in the GO and KEGG annotations were also upregulated inmdxHRTΔIKKβhearts (Fig.2a). This is in sharp contrast to microarray results we recently reported from Ras transformedp65−/−mouse embryonic fibroblasts (MEFs), which ...
“significant” status. The resulting P-values were adjusted using the Benjamini and Hochberg’s approach for controlling the false discovery rate. Genes with an adjusted P-value of < 0.05 were assigned as differentially expressed. The Gene Ontology and KEGG analyses of the differentially ...