近年来新兴的纳米孔测序技术能够对长链RNA直接测序（nanopore direct RNA-sequencing），无需 PCR 扩增，可以同时在核苷酸序列中识别碱基修饰【3,4】。2021年7月19日，来自于新加坡A*STAR基因研究所的Jonathan Göke 研究组在Nature Biotechnology杂志上发表了题为Identification of differential RNA modifications from ...
不同于前两代测序技术,第三代纳米孔测序技术无需PCR扩增,利用纳米孔特殊结构实现单条DNA/RNA分子的实时测序,从而克服了第二代测序技术(NGS)中存在的PCR扩增误差、测序读长短、DNA/RNA甲基化修饰的问题,并广泛应用于基因组测序、突变鉴定等多个研究领域。 01 【产品简介】 Oxford Nanopore Sequencing第三代测序系统...
Quantitative profiling of N 6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing[J]. Genome biology, 2021, 22: 1-17. ^Kovaka S, Hook P W, Jenike K M, et al. Uncalled4 improves nanopore DNA and RNA modification...
21号,BASE团队在Nature Communications发表文章:mRNA vaccine quality analysis using RNA sequencing,他们开发了一种采用长读长纳米孔测序分析 mRNA 疫苗关键质量属性的方法,称为 VAX-seq,对 mRNA 序列,长度,完整度,纯度,修饰核苷展开精确定量分析。相比现有昂贵耗时的分析方法(HPLC、毛细管电泳、凝胶电泳等),VAX-seq ...
文章名称:Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome 发表期刊:Plant Method 发表时间:2020年4月 影响因子:3.610 研究内容:在许多不同的研究领域中,使用Pacifc Biosciences(PacBio)和牛津纳米孔技术(ONT)进行第三代测序的研究数量正在迅速增加。
然而二代RNA测序技术存读长较短,依赖mRNA反转录和PCR扩增,系统偏向性的缺陷。近年来新兴的纳米孔测序技术能够对长链RNA直接测序(nanopore direct RNA-sequencing),无需 PCR 扩增,可以同时在核苷酸序列中识别碱基修饰【3,4】。 2021年7月19日...
However, these methods lose information contained in biological RNA because the copied reads are often short and because modifications are not carried forward in cDNA. We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies (ONT). Our ...
文章名称:Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis tranome 发表期刊:Plant Method 发表时间:2020年4月 影响因子:3.610 研究内容:在许多不同的研究领域中,使用Pacifc Biosciences(PacBio)和牛津纳米孔技术(ONT)进行第三代测序的研究数量正在迅速增加。与...
结果表明,nanopore direct RNA-seq/xPore鉴定的m6A位点与基于二代测序的m6ACE-seq(m6A-cross- linking-exonuclease sequencing )结果高度一致,90% xPore鉴定出的显著性m6A位点(top significant 1500 positions)与DRACH motif(D=G/A/U, R=G/A, H=A/U/C,最常见的m6A motif)或者其他已知m6A位点高度重合。为...
RNA sequencing with nanopore technology Learn more Single-cell sequencing with Oxford Nanopore Learn more Recommended for you Related areas View all applicationsHow to get started with nanopore sequencingHow to extract high-quality DNA and RNAHow to select the right library prep workflow...