Direct RNA 测序 全长lncRNA测序 TAIL Iso-seq 全长转录本Reference图谱 Long non-coding RNAs测序 多组学研究方案 普通转录组测序 微生物多样性 抗生素抗性基因分析 全长宏基因组 全长微生物多样性 二代微生物多样性 医学服务 MEDICAL SERVICES 病原微生物检测 TNPD®常见感染病原检测 TNPD®血流感染病原...
结果表明，nanopore direct RNA-seq/xPore鉴定的m6A位点与基于二代测序的m6ACE-seq （m6A-cross- linking-exonuclease sequencing ）结果高度一致，90% xPore鉴定出的显著性m6A位点（top significant 1500 positions）与DRACH motif (D=G/A/U, R=G/A, H=A/U/C，最常见的m6A motif) 或者其他已知m6A位点高度...
结果表明,nanopore direct RNA-seq/xPore鉴定的m6A位点与基于二代测序的m6ACE-seq(m6A-cross- linking-exonuclease sequencing )结果高度一致,90% xPore鉴定出的显著性m6A位点(top significant 1500 positions)与DRACH motif(D=G/A/U, R=G...
这项研究揭示了mTOR和LARP1通过调控TOP mRNA的poly(A)尾长度来影响其翻译的新机制。它不仅加深了我们对细胞翻译调控的理解,还为研究RNA在细胞生理和疾病中的作用提供了新的思路。 DRS技术的前景 DRS技术在这篇文章中展现出的高精度和高信息量的RNA分析能力,无疑是它的一大亮点。对于想要深入研究RNA分子特征和转录...
Since the direct-RNA sequencing method sequences from the 3’end to the 5’end of the RNA, the poly(A) information is completely preserved during the process of adding the poly(T) adapter. Thus, an estimate of the RNA poly(A)tail legnth can be read from the electrical signal of the ...
direct rna sequencing - sequence-specific (sqk-rna004) pack size 6 reactions stability shipped at 2–8°c long-term storage -20 – -30°c the warranty for this product is 3 months from receipt by the customer the direct rna sequencing kit (sqk-rna004) is used to prepare and ...
本周一,Nature Methods在线发表Oxford Nanopore direct RNA测序技术测评文章,结果表明,【不经反转、无须扩增的RNA直接测序】能获得全长的链特异性RNA,无测序偏好性,并同时记录碱基修饰,为后续研究基因结构和基因表达,提供新技术新方法。 direct RNA建库测序
Sequencing for RNA modifications with the nanopore direct RNA sequencing platform provides ionic current levels, helicase dwell times, and base call data that differentiate the modifications from the canonical form. Herein, model RNAs were synthesized with site-specific uridine (U) base modifications ...
普通RNA-seq需要考虑建库过程中的artifect而Nanopore可以直接检测到antisense transcripts 普通RNA-seq需要在合成cDNA的基础上进行PCR扩增建库而Nanopore直接对RNA进行阅读,所以可以保留splice junction,RNA修饰等信息 Nanopore可以阅读long-read RNA Nanopore不需要enzymatic synthesis reaction 2. RNA修饰是什么 RNA修饰指的是...
图1 使用xPore从Direct RNA-seq数据中定量RNA修饰 主要结果 xPore单碱基分辨率鉴定m6A位点 作者首先将该方法运用于野生型(WT)HEK293T细胞(“WT细胞”)和通过CRISPR-Cas9敲除m6A writer METTL3的细胞(“敲除(KO)细胞”)。各进行3次重复,总共产生了近800万条reads。使用超过900万个位点进行建模,过滤后有939,902个...