219Citations 180Altmetric Metrics Abstract Inspired by the biological processes of molecular recognition and transportation across membranes, nanopore techniques have evolved in recent decades as ultrasensitive analytical tools for individual molecules. In particular, nanopore-based single-molecule DNA/RNA seque...
The invention discloses a detection method for biomolecular probe-calibrated specific sites of DNA based on a nanopore device and application thereof. The method comprises the following steps: 1) calibrating the specific sites of DNA by using a biomolecular probe so as to obtain calibrated DNA; ...
For example, a biological nanopore-based direct, digital counting of single-nucleotide poly- morphic sites marked with locked nucleic acid–synthetic molecules was used for the detection of Shiga toxin-producing Escherichia coli serotype and cancer-derived driver mutations35,124. Another approach using ...
Nanochannel Device with Embedded Nanopore: a New Approach for Single-Molecule DNA Analysis and ManipulationNanopore and nanochannel based devices are robust methods for biomolecular sensing and single DNA manipulation. Nanopore-based DNA sensing... Y Zhang,W Reisner - 《American Physical Society》 被引...
Biological nanopore approach has proved successful for single﹎olecule DNA sequencing, which resolves the identities of 4 natural deoxyribonucleotides based on the current blockages and duration times of their translocations across the nanopore confinement. However, open challenges still remain for ...
simplicity, label-free, and solution-based detection, therefore widely used in analytical detection [[33], [34], [35], [36], [37], [38], [39], [40], [41], [42]], DNA sequencing [43,44], biomolecular interaction [45,46], and protein unfolding [[47], [48], [49], [50]]...
12 August 2014 - UK nanopore-based electronic systems developer OxfordNanoporeTechnologies Ltd unveiled today it had secured GBP35m (USD59m/EUR 44m) in new investment via a private share placement. Oxford Nanopore raises GBP35m As a complex organic material with a complicated porous structure, coal...
7 has been impeded by these challenges. Solving the time and selectivity challenges would open the path to a new generation of nanopore-based biomolecular quantitative analysis systems6,8,9. These would provide single molecule resolution, could be calibration-free as they rely on counting alone10...
Nanopore-based sensors have established themselves as a prominent tool for solution-based, single-molecule analysis of the key building blocks of life, including nucleic acids, proteins, glycans and a large pool of biomolecules that have an essential role in life and healthcare. The predominant mol...
Described herein are nanopore biosensors based on a modified cytolysin protein. The nanopore biosensors accommodate macromoiecules including proteins and nucleic acids, and may addi