更重要的是,WT B 细胞最终变得独立于自身反应性 B 细胞,能够在自身反应性 B 细胞缺失的情况下维持 GC 反应。 2024年3月22日,美国哈佛医学院Michael C. Carroll在Science Immunology上发表题为“Invasion of spontaneous germinal centers by naive B cells is rapid and persistent”的文章。本研究证实自身反应性G...
Naïve B cells provide an additional path for delivery of IC into the B cell follicles and retention on FDC [31••]. Immune complexes are bound by SCS macrophages and in the presence of complement C3 transferred to naïve B cells in the underlying follicles [31••]. Subsequently...
初始B细胞富集试剂盒II用于从人的PBMC中快速有效地分离未经标记的初始B细胞。 详细的分离过程 非B细胞(T细胞,NK细胞,树突状细胞,单核细胞,粒细胞,血小板和类红细胞)和非初始B细胞(记忆B细胞,活化B细胞和浆细胞)被生物素化抗体混合物 CD2,CD14,CD16,CD27,CD36,CD43和CD235a(糖蛋白A)标记。 这些细胞随后用抗...
Within the germinal center, B cells compete for a limiting amount of T cell-derived signals that are necessary for their continued proliferation, somatic hypermutation, and eventual differentiation into memory B cells or plasma cells (Laidlaw & Cyster, 2021). Memory B cells and plasmablasts can ...
MACSxpress全血初始B细胞富集试剂盒用于从抗凝全血中分离未经标记的初始B细胞,无需密度梯度离心。 详细的分离过程 借助新的MACSxpress®技术,可以从多达30 mL的抗凝全血中分离未经标记的人白细胞亚群。借助MACSxpress磁珠, 通过免疫磁珠去除非靶细胞。 同时沉降红细胞,分选未经标记的高纯度靶细胞。
Giordani L, Sanchez M, Libri I, Quaranta MG, Mattioli B, Viora M (2009) IFN-{alpha} amplifies human naive B cell TLR-9-mediated activation and Ig production. J Leukoc Biol 86:261–271Giordani L, Sanchez M, Libri I, Quaranta MG, Mattioli B, Viora M. IFN-alpha amplifies human naive...
The Naive B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched naive B cells from human PBMCs. Detailed separation procedure Non-B cells (T cells, NK cells, dendritic cells, monocytes, granulocytes, platelets, and erythroid cells) and non-naive B cells (mem...
We have identified a distinct pre-naive B cell population circulating in human peripheral blood that exhibits an intermediate phenotype between transitional and naive B cells. Like human transitional B cells, these cells express CD5 but have intermediate densities of CD38, CD10, CD9, and the ABCB...
什么是Naïve B cell 与T细胞类似,B细胞也是从干细胞分化而来,通过接触独特的抗原分化后激活。B细胞表面受体BCR与外来抗原结合后发挥下游生物学功能。通常来说,B细胞发育的最后阶段在脾脏中,在那里发育成初始B细胞。这些不被激活的初始B细胞随着血液进入到刺激淋巴器官(SLO)。初始的B细胞更容易捕获病原体,因为SLO的...
Deep analysis of B cell activation pathways by our group and others has led to a strong emphasis on the extrafollicular (EF) pathway as a common feature of severe disease14,15,17. Characterized by the induction of T-bet-driven double-negative 2 (CD27−, IgD−, CD11c+, CD21−(DN2...