更重要的是,WT B 细胞最终变得独立于自身反应性 B 细胞,能够在自身反应性 B 细胞缺失的情况下维持 GC 反应。 2024年3月22日,美国哈佛医学院Michael C. Carroll在Science Immunology上发表题为“Invasion of spontaneous germinal centers by naive B cells is rapid and persistent”的文章。本研究证实自身反应性G...
Naïve B cells provide an additional path for delivery of IC into the B cell follicles and retention on FDC [31••]. Immune complexes are bound by SCS macrophages and in the presence of complement C3 transferred to naïve B cells in the underlying follicles [31••]. Subsequently...
初始B细胞富集试剂盒II用于从人的PBMC中快速有效地分离未经标记的初始B细胞。 详细的分离过程 非B细胞(T细胞,NK细胞,树突状细胞,单核细胞,粒细胞,血小板和类红细胞)和非初始B细胞(记忆B细胞,活化B细胞和浆细胞)被生物素化抗体混合物 CD2,CD14,CD16,CD27,CD36,CD43和CD235a(糖蛋白A)标记。 这些细胞随后用抗...
MACSxpress全血初始B细胞富集试剂盒用于从抗凝全血中分离未经标记的初始B细胞,无需密度梯度离心。 详细的分离过程 借助新的MACSxpress®技术,可以从多达30 mL的抗凝全血中分离未经标记的人白细胞亚群。借助MACSxpress磁珠, 通过免疫磁珠去除非靶细胞。 同时沉降红细胞,分选未经标记的高纯度靶细胞。
Within the germinal center, B cells compete for a limiting amount of T cell-derived signals that are necessary for their continued proliferation, somatic hypermutation, and eventual differentiation into memory B cells or plasma cells (Laidlaw & Cyster, 2021). Memory B cells and plasmablasts can ...
IFN-alpha amplifies human naive B cell TLR-9-mediated activation and Ig production. J Leukoc Biol. 2009; 86 :261–71. [ PubMed ]Giordani L, Sanchez M, Libri I, Quaranta MG, Mattioli B, et al. (2009) IFN-alpha amplifies human naive B cell TLR-9-mediated activation and Ig production...
The perspective that naive B-cell recognition of antigen in the absence of T-cell help causes cell death or anergy is supported by in vivo studies of B cells that are continuously exposed to self-antigens. However, intravital imaging suggests that early
什么是Naïve B cell 与T细胞类似,B细胞也是从干细胞分化而来,通过接触独特的抗原分化后激活。B细胞表面受体BCR与外来抗原结合后发挥下游生物学功能。通常来说,B细胞发育的最后阶段在脾脏中,在那里发育成初始B细胞。这些不被激活的初始B细胞随着血液进入到刺激淋巴器官(SLO)。初始的B细胞更容易捕获病原体,因为SLO的...
Thus, influenza virus vaccination in humans can elicit a germinal centre reaction that recruits B cell clones that can target new epitopes, thereby broadening the spectrum of vaccine-induced protective antibodies. This is a preview of subscription content, access via your institution Access options ...
初始T细胞(naive T cell)是未接触过抗原的成熟T淋巴细胞,由骨髓干细胞在胸腺中经历复杂发育过程形成,其功能状态和数量受pre-TCRα等分子调控。以下从定义、发育机制、相关分子机制及人类临床案例展开分析。 一、初始T细胞的生物学定义与起源 初始T细胞起源于骨髓干细胞分化形成的T淋巴母细胞...