a, tSNE plot showing unsupervised clusters based on scRNA-seq gene expression of cells in the ‘B cell’ cluster of Extended Data Fig. 2f, pooled from all samples and time points from participant 05. b, Dot plot showing the average log-normalized expression of a set of marker genes and...
The splenic marginal zone of adult humans contains B cells, of which most express CD27, an antigen only recently identified as a marker for somatically mutated memory B cells. We investigated whether and to which extent the developing marginal zone in infants and children is populated by either...
Germinal center B cells and plasmablasts were then measured to assess the quality of the primary response generated in each strain. The GL-7 marker was used to identify germinal center cells and abundant intracellular Ig was used to identify plasmablasts. The frequency and total numbers of PE-...
spontaneously differentiate into IgM-secreting cells and be responsible for the production of ‘natural antibodies.’ The phenotype of these cells is similar to the one of MZ B cells, with the addition of CD43 surface expression (a plasma cell marker), and it has been proposed accordingly that...
In humans, CD27 is a good marker for plasmacells,naiveBcellsare CD27-, memory B-cells are CD27+ and plasma cells are CD27++. WikiMatrix B-cells that have not encountered an antigen are callednaiveBcells. WikiMatrix Some cells are specially equipped to present antigen, and to primenaive...
The perspective that naive B-cell recognition of antigen in the absence of T-cell help causes cell death or anergy is supported by in vivo studies of B cells that are continuously exposed to self-antigens. However, intravital imaging suggests that early
The recognition that CD27 expression among peripheral human B cells provides a robust distinguishing marker for the memory pool has proved invaluable for dissecting the behavior of this subset and comparing it with the naive IgD+ population 25. Importantly, the reciprocal expression of IgD and CD...
B cells (1382 cells; 4.48%; marked with CD79A),; nerve cells (1251 cells; 4.05%; with marker gene NGFR),; mast cells (870 cells; 2.82%; with marker gene TPSB2),; umap的可视化 如下所示: umap的可视化 值得注意的是在癌旁正常组织的图谱里面,都是naive的CD4和CD8阳性T细胞。但是在肿瘤组织...
According to the professor, why are some cells called "naive"? A. They originate from a relatively primitive type of cell. B. They are easily eliminated by the immune system. C. They are not yet able to recognize any particular protein marker. ...
After staining, cells were washed with 1 ml FACS buffer and resuspended in 300 μl FACS buffer for surface marker analyses. The frequency and phenotype of DNA tetramer-binding B cells were detected by the tetramer staining technique, resuspended in 100 μl FACS buffer, and incubated ...