Interestingly, we also found that CpG ODN induces naive B cell maturation into memory cells, as demonstrated by the induction of CD27, AID mRNA expression, and IgG production. More importantly, our results demonstrate that IFN-a amplifies the inductive effect of CpG ODN on naive B activation ...
Among the many signals naive B cells receive, the engagement of the B cell receptor by foreign antigens and cytokines secreted by T cells are critical for B cell activation and differentiation. B cells undergo class switching to achieve a diverse array of effector functions and produce various ...
Hence the activation ofnaiveTcellsis crucially dependent on adequate calcitriol levels. WikiMatrix NaiveTcellshave very low expression of VDR and PLC-γ1. WikiMatrix Initially,naiveBcellsexpress only cell-surface IgM and IgD with identical antigen binding regions. ...
Deep analysis of B cell activation pathways by our group and others has led to a strong emphasis on the extrafollicular (EF) pathway as a common feature of severe disease14,15,17. Characterized by the induction of T-bet-driven double-negative 2 (CD27−, IgD−, CD11c+, CD21−(DN2...
In addition, in cooperation with helper T cells in response to antigen stimulation, it could enchance autoreactive naive B cell activation and the process of somatic hypermutation in germinal center. Due to defective selection process by follicular dendritic cells (FDCs), memory B cells might ...
interferon lambda JAK-STAT signaling B cell activation phosphorylation mTORC1 pathway cell cycle plasmablast differentiation immune modulation transcriptomics effector function Introduction Type III interferons (IFNs), or the interferon lambda (IFN-λ) family, are crucial antiviral effectors. IFN-λ-mediated...
(Fig. 2a–h). Of interest, we found that Ig-Tg B cells formed GC, memory and PCs even at antigen doses suboptimal for B-cell activation (based on their failure to induce CD69 and CD86 upregulation or surface IgM downregulation,Fig. 2a–d). However, significantly decreased accumulation ...
In addition, in cooperation with helper T cells in response to antigen stimulation, it could enchance autoreactive naive B cell activation and the process of somatic hypermutation in germinal center. Due to defective selection process by follicular dendritic cells (FDCs), memory B cells might ...
Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines. Nat Biotechnol. 2002;20(11):1129–34. Article CAS Google Scholar Delker RK, Fugmann SD, Papavasiliou FN. A coming-of-age story: activation-induced cytidine deaminase turns 10. Nat Immunol. ...
CD38, Ki-67, CD95 and bcl-2 were used as activation, proliferation and apoptotic markers. Susceptibility to apoptosis was evaluated by cell size and annexin-V binding in culture cells. The percentage of CD27+ B cells was significantly lower in aged (mean, 19.2%) individuals than that in ...