目的探讨SREBP1c对mGLUT1表达的影响。方法采用分子克隆技术,将SREBP1c和mGLUT1cDNA分别克隆到表达载体pSVsport和pGL3b上。SREBP1c与mGLUT1克隆载体同时或单独转染NIH3T3细胞,然后应用荧光素酶活性测定法、RTPCR及Northernblot等方法测定SREBP1c对mGLUT1的启动子活性调节及mRNA表达的影响。结果实验结果表明,SREBP1c可...
目的: 探讨PNPLA3基因I148M过表达Huh7细胞中SREBP-1c与甘油三酯的关系.为非酒精性脂肪肝的发病机制研究提供线索. 方法: 用空病毒和携带PNPLA3基因I148M突变型的慢病毒感染Huh-7细胞,构建空病毒组(对照组)和PNPLA3基因I148M突变型组(突变组).分别用油红O染色显示细胞内甘油三酯含量变化,全自动生化分析仪测定...
By performing detailed molecular characterization in the livers of these mice, we also determined the mechanism responsible for the increase in hepatic fat in these animals, which was correlated with the processing of liver SREBP-1c. Materials and Methods Animals Mice harboring Mboat7 floxed alleles...
目的 探讨柴胡加龙骨牡蛎汤对肝郁大鼠肝脏脂质代谢及固醇调节元件结合蛋白-1c/脂肪酸合酶(SREBP-1c/FAS)信号通路的影响.方法 将50只雄性SPF级SD大鼠随机分为空白组,模型组,氟西汀组,柴胡加龙骨牡蛎低剂量组,柴胡加龙骨牡蛎高剂量组,每组10只.空白组正常喂养,其余组大鼠采用慢性温和不可预知应激(CUMS)方法建立肝...