为保证最大敲除效率,SREBP-1 CRISPR/Cas9 敲除质粒 (m) 包含三个质粒,每个质粒都编码Cas9 核酶和 SREBP-1-特异性20nt向导RNA. gRNA 序列来源于 GeCKO (v2)文库,指导Cas9蛋白去诱导基因组DNA双链中一个特异位点的断裂。 如果需要筛选Cas9成功诱导双链断裂的细胞,推荐用 SREBP-1 HDR质粒(m) (sc-423152-HDR)...
然而在蛋白水平上,TRIM21敲除增加了脂质代谢相关基因的表达,包括SREBF1、FASN、GPAM、ATGL、perilipin-1和MGLL(图4C),过表达TRIM21降低了这些基因的表达(图4D)。 SREBF1主要调控参与脂肪酸、甘油三酯和磷脂合成的基因。CERS6和Lipin-1被SRE...
By performing detailed molecular characterization in the livers of these mice, we also determined the mechanism responsible for the increase in hepatic fat in these animals, which was correlated with the processing of liver SREBP-1c. Materials and Methods Animals Mice harboring Mboat7 floxed alleles...
目的 探讨柴胡加龙骨牡蛎汤对肝郁大鼠肝脏脂质代谢及固醇调节元件结合蛋白-1c/脂肪酸合酶(SREBP-1c/FAS)信号通路的影响.方法 将50只雄性SPF级SD大鼠随机分为空白组,模型组,氟西汀组,柴胡加龙骨牡蛎低剂量组,柴胡加龙骨牡蛎高剂量组,每组10只.空白组正常喂养,其余组大鼠采用慢性温和不可预知应激(CUMS)方法建立肝...
硒元素对非酒精性单纯性脂肪肝大鼠PPARα、C/EBPα、SREBP-1c及SREBP-2的影响 结论硒元素可通过纠正血脂紊乱,改善肝功能和肝细胞脂肪变性防治单纯性脂肪肝,其作用机制可能与上调PPARα,下调C/EBPα,SREBP-1c m RNA表达,提高肝总脂酶活性,... 樊秀琴,杨青,金杭斌,... - 《中华全科医学》 被引量: 0发表...
Knockdown of AMPKα1 or/and AMPKα2 repressed Rspos-induced inhibition on cholesterol synthesis. Our study indicates that Rspo1/Rspo3-LGR4 signaling in hepatocytes suppresses cholesterol synthesis via the AMPKα-SREBP2 pathway. 展开 关键词: AMPK cholesterol LGR4 liver R‐spondins ...