This study adapts the MS2-MCP system to visualize endogenous mRNAs in Caenorhabditis elegans. Inactivating nonsense-mediated decay and expressing low levels of cytoplasmic MCP allows the live imaging of functional, cytoplasmic transcripts in the nematode.Tocchini, Cristina...
在crRNA介导下,融合表达荧光蛋白的dCas13蛋白靶向RNA,实现RNA成像. CRISPR: 成簇的、规律间隔的短回文重复序列; crRNA:CRISPR RNA;dCas:失活的Cas. Figure 4 Schematic diagram of CRISPR/dCas13 system 1.2 荧光染料由于灵敏度高、操作方便,逐渐取代放射性同位素作为检测标记广泛应用于荧光探针和细胞染色等。荧光RN...
MS2 systemMS2V6MS2V7Quantitative fluorescence microscopyShort-lived mRNAsSingle cellSingle-molecule imagingsmFISHVisualization of single mRNAs in their native cellular environment provides key information to study gene expression regulation. This fundamental biological question triggered the development of the.....