The RNA is detected by the co-expression of fluorescent protein-tagged MS2 CP (MCP), which binds to each of the MSL elements. In providing a detailed protocol for the in vivo visualization of TMV-MP mRNA tagged with the MS2 system in Nicotiana benthamiana epidermal cells, we describe (1)...
Expression vectors for MS2 coat protein (MCP) and ADAR1 deaminase domain (ADAR1 DD) were also prepared in previous work [30]. In the present study, MCP harbored an asparagine to lysine mutation at amino acid position 55 (N55K) because the mutation has higher binding affinity to MS2 RNA ...