融合表达荧光蛋白的MCP二聚体与MS2茎环结合实现RNA成像. MCP:MS2衣壳蛋白. Figure 1 Schematic diagram of MS2/MCP system 1.1.2 Larson等[13]基于假单胞菌RNA噬菌体的PP7开发了PP7/MCP系统(图2),其标记RNA的原理与MS2/PCP系统相同。噬菌体PP7的外壳蛋白与其同源RNA结构具有较强的亲和力[34]。研究者使用PP7...
MS2-MCP systemMammalian cellsS. cerevisiaeSingle cellSingle moleculeTranscriptionmRNA labelingmRNA localizationLive imaging of single RNA from birth to death brought important advances in our understanding of the spatiotemporal regulation of gene expression. These studies have provided a comprehensive ...
The MS2–MCP system enables researchers to image multiple steps of the mRNA life cycle with high temporal and spatial resolution. However, for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, ...
10.1074/mcp.T400006-MCP200 Article Google Scholar Bradshaw RA, Burlingame AL, Carr S, Aebersold R: Reporting Protein Identification Data: the next generation of guidelines. Molecular & Cellular Proteomics 2006, 5.5: 787-788. 10.1074/mcp.E600005-MCP200 Article Google Scholar Fenyo D, Beavis ...
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This study adapts the MS2-MCP system to visualize endogenous mRNAs in Caenorhabditis elegans. Inactivating nonsense-mediated decay and expressing low levels of cytoplasmic MCP allows the live imaging of functional, cytoplasmic transcripts in the nematode.Tocchini, Cristina...
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved ...
Live imaging and quantitation of nascent transcription using the MS2/MCP system in the Drosophila embryoGene expressionMicroscopyModel organismsMolecular biologyVisualizing transcription live in Drosophila is providing important new insights into the spatiotemporal regulation of transcription. Here, we describe ...
Note: In addition to the MS2/MCP system, an orthogonal mRNA tagging system, based on the Pseudomonas phage derived PP7 stem-loops and respective PCP coat protein, can be used to visualize nascent transcription (Chao et al., 2008; Pichon et al., 2018). Show abstract A rapid inducible RNA...
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved ...