Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Cefoxitin is a potent inducer of the i mecA /i regulatory system. It is being recommended for detection of methicillin resistance in i Staphylococcus aureu... KB Anand,P Agrawal,S...
Real-time PCRStaphylococcus aureusmecA genemecC geneTraditional antimicrobial susceptibility test methods for detection of S. aureus resistant to oxacillin (MRSA) such as disk diffusion, broth microdilution, and oxacillin screen plate require 18鈥 24 h of incubation after having the organism growing in...
To screen genomes for copy number variation, we used the cn.MOPS R package61 using the haplocn.mops algorithm adjusted for haploid genomes. All genomes were screened visually for regions with potentially changed copy number. Next, the mean read count per base was generated and an R script ...
Staphylococcus aureus(SA) is a common skin bacterium. It is sometimes called staph, and it most often causes skin and soft tissue infections. AlthoughS. aureushas been causing staph infections as long as humans have existed, MRSA has only been around since 1961. Methicillin was one of the fi...
The present invention provides multiplex assays, methods and kits that may be used to detect and confirm the presence of MRSA in a sample. The methods include real-time PCR assays,
clearlyincreased incidence of MRSA (G2/100 patients orG0.3MRSA infections/100 patients) or in case of difficulties inimplementing risk-oriented screening, a general screen-ing of all patients at admission, for example, to the inten-sive care unit might possibly be a good policy and becost-...
株,用多重PCR方法进行SCCmec分型;并进行回顾性研究,分析PVL基因阳性及阴性株所致感染的l临床特点及相关危险因 素;用琼脂稀释法测定其对l临床常用抗菌药的敏感性(MIC)。结果 在1366株MRSA菌株中共筛出5株PVL基因阳性菌 株,阳性率为0.37 。50株MRSA临床分离菌株中,SCCmecI型1株,占2 ...
Wassenberg MW, Kluytmans JA, Box AT et al (2010) Rapid screening of methicillin-resistant Staphylococcus aureus (MRSA) using PCR and chromogenic agar: a prospective study to evaluate costs and effects. Clin Microbiol Infect doi:10.1111/j.1469-0691.2010.03210 Google Scholar WHO guidelines on ha...
This was carried out according to NCCLS guidelines using Oxacillin agar screen test whereby all MRSA isolates were spot inoculated onto Mueller-Hinton agar supplemented with 6 μg/ml oxacillin and 4% NaCl, from a 0.5 McFarland standard suspension. The plates were incubated at 35°C for 24 h ...
Real-time PCRChromogenic agarAntimicrobial susceptibility test methods such as disk diffusion, broth microdilution, and oxacillin screen plate require 24 h of incubation after having the organism growing in pure culture. Rapid and accurate identification of MRSA isolates is essential not only for patient...