The previous identification step may lead to false positives. Thus, the presence of MRSA is eventually validated by molecular analysis usingPCR (Polymerase Chain Reaction). Once validated, molecular typing completes the strain characterization. A subset of representative MRSA isolates can be selected bas...
PCR) in a blood sample in as little as two hours. The test is able to determine whether the genetic material is from MRSA or from less resistant forms of staph bacteria. It may allow hospitals to start precautions early. It may also allow doctors to quickly tailor ...
表2 多重 PCR 所选取的扩增位点及其定位 Table 2 Location and meaning of loci used in the multiplex PCR Amplicon sizeΠbp Locus Position Meaning Specific locus of type2Ⅰ Specific locus of type2Ⅱ Shared by type2Ⅱ, Ⅲ Shared by type2Ⅰ,Ⅱ, Ⅳ Specific locus of type2 Ⅲ Specific locus...
Minocycline MIC ranged from 0.032 to 8 μg/mL, meaning that all were susceptible except 3 isolates which were intermediate (8 μg/mL). They showed 2 peaks, one at 0.094 – 0.125 μg/mL and the other at 2 – 3 μg/mL. Gatifloxacin MICs for susceptible strains ranged from 0.016 to ...
Minocycline MIC ranged from 0.032 to 8 μg/mL, meaning that all were susceptible except 3 isolates which were intermediate (8 μg/mL). They showed 2 peaks, one at 0.094 – 0.125 μg/mL and the other at 2 – 3 μg/mL. Gatifloxacin MICs for susceptible strains ranged from 0.016 to ...
Genome sequencing is revolutionizing clinical microbiology and our understanding of infectious diseases. Previous studies have largely relied on the sequencing of a single isolate from each individual. However, it is not clear what degree of bacterial di