异质性来源于MRSA筛查时使用的鉴定方法(PCR法/细菌培养法)。Deeks漏斗图显示,纳入研究不存在发表偏倚。结论 MRSA鼻腔筛查对于筛查MRSA手术部位感染具有较高的特异度和阴性预测值,可用于排除MRSA肺炎,患者鼻腔未定植MRSA,则发展为MRSA手术部位感染的可能性较小。所以,MRSA鼻腔筛查可作为规范抗菌药物经验性用药的一种有效...
方法 收集临床 64 株葡萄球菌标本 ,对其耐药标志基因 mecA 基因和金黄色葡萄球菌特有基因femA基因扩增后应用压电传感器阵列进行检测 ,并以 PCR 电泳检测作参照 ,与目前采用的常规药物敏感试验进行对比分析。结果 传感器分析与 PCR 电泳分析结果完全一致。而此两种方... ...
PCRScreeningCritical careWe report a significant reduction in the rate of meticillin-resistant Staphylococcus aureus (MRSA) transmission on a critical care unit when admission screening by culture was replaced with a same-day polymerase chain reaction (PCR) test. This was an observational cohort...
in64collected strains of staphylococcus and the results were com pared with those obtained by PCR and conventional antimicrobial susceptibility test.Re sults The results by sens or arrays were identical to those by PCR,but had s ome differences with those by antimicrobial susceptibility test.Am ...
as S. aureus based on morphology, coagulase test, DNase test and mannitol salt agar fermentation. Disk diffusion antibiotic susceptibility tests were performed according to the guidelines of the Clinical and Laboratory Standards Institu...
Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher th...
[摘要] 目的 观察实时荧光PCR检测耐甲氧西林金黄色葡萄球菌(MRSA)对减少儿科ICU病区医院感染的效果。方法 选取2011-07～2012-06实时荧光PCR法检测的儿科ICU病区标本856份,其结果与分离培养药敏试验法的检测结果进行比较;另与2009-07～2010-06 MRSA医院感染率进行比较。结果 实时荧光PCR法与分离培养药敏试验法对金葡...
MRSA临床介绍 超级细菌（MRSA）防控与实验室检测 程洪波上海之江生物 内容提要 MRSA的流行病学 MRSA的防控指南 MRSA的检测方法 MRSA 葡萄球菌 金黄色葡萄球菌MRSA 金葡/表葡/腐生 致病性强 耐甲氧西林金葡 •• MRSA定义：凡对甲氧西林、苯唑西林、头孢西丁耐药，或青霉素结合蛋白2a（PBP2a）阳性、mecA基因阳性...
Roche Molecular Diagnostics(Pleasanton, CA) offers the LightCycler®MRSA Advanced Test. The test is performed on the LightCycler 2.0 instrument and uses swab extraction and mechanical lysis for specimen preparation followed by RT-PCR, and fluorogenic target-specific hybridization probes for detection of...
目的:评价Vitek 2 AST P535药敏卡片测定金黄色葡萄球菌对苯唑西林敏感性的准确性.方法:对临床分离的菌株采用AST P535药敏卡片和头孢西丁纸片测定对苯唑西林的耐药性,以PCR测定MecA基因为金标准,评价两种方法的准确性.结果:采用PCR共检测125株金黄 色葡萄球菌,其中MecA基因阳性的耐甲氧西林金黄色葡萄球菌(Methicillin ...