Nasal swab PCRPneumoniaBACKGROUND :Methicillin-resistant Staphylococcus aureus (MRSA) nasal swab polymerase chain reaction (PCR) assay has a 96.1-99.2% negative predictive value (NPV) in pneumonia and may be used for early de-escalation of MRSA-active antibiotic agents. Xu (2018), File (2010) ...
Roche Molecular Diagnostics(Pleasanton, CA) offers the LightCycler®MRSA Advanced Test. The test is performed on the LightCycler 2.0 instrument and uses swab extraction and mechanical lysis for specimen preparation followed by RT-PCR, and fluorogenic target-specific hybridization probes for detection of...
Bacterial isolation and antibiotic susceptibility from diabetic foot ulcers in Kenya using microbiological tests and comparison with RT-PCR in detection of S. aureus and MRSA.BMC Research Notes.2019;12(1):244. Gherardi G, Angeletti S, Panitti M, Pompilio A, Di Bonaven- tura G, Crea F, et...
Clinical utility of a nasal 通培养法性能更好,且用时更短.但 PCR 方法价格 swab methicillin- resistant Staphylococcus aureus polymerase chain 更高,每个样本需要 26 美元(约合人民币 180 元),普 通培养法仅需 7 美元(约合人民币 50 元)[11].PCR 法 可在两小时内为终止抗 MRSA 治疗提供依据,而应用 ...
Methods: Admission or presurgical nursing queries identified patients that met criteria for a nares swab to detect MRSA colonization. Swabs were obtained either at the presurgical visit or at admission and tested by BD CHROMAgar or GeneOhm PCR methods. The majority of labs performed agar testing...
Furthermore, an isolate from staff member F (Staff_F_1_T) was directly basal to one of the two sub-clades from staff member A's third swab (Fig. 5a). Dog 150, which was both nasally colonized and had wound infected by clade 2 isolates, first entered the veterinary hospital on day...
Provided that there is effi-cient DNA preparation from the swab, a well functioningPCR protocol and a solid evaluation performed on a rep-resentative spectrum of clinical specimens, this methodnormally can achieve a negative predictive value of morethan 99% for the direct detection of MRSA (mecA...
Table 2. Prevalence ofS. aureusand MRSA in different types of samples by PCR. SourcesNumber of Taken sampleS. aureus %MRSA% Raw Milk263107 (40.7%)31(11.8%) Cow nasal swab263123(46.8%)35(13.3%) Human nasal swab5317 (32.1%)8(15.1%) ...
Methods: We determined the rate of patients remaining free of MRSA after being cleared based on 2 negative nasal PCR screens at least 1 week apart or 1 negative PCR if the last positive was >1 year ago, without concurrent antibiotics. All nasal screening results (PCR and agar) and clinical...
RT-PCR assay was performed for analysis of 72 nasal swab specimens, and the results were confirmed by use of a culture method. Furthermore, the results of RT-PCR were compared with LightCycler MRSA advance test. The multiplex RT-PCR assay reproducibly detected a minimum of 1pg genomic DNA (...