外周血单个核细胞(Peripheral blood mononuclear cell,PBMC)是指外周血中具有单个细胞核的细胞,包括淋巴细胞,单核细胞等。 提取方法: 全血样本聚蔗糖-泛影葡胺(Ficoll-Hypaque)密度梯度离心分离 外周血单个核细胞(Peripheral Blood Mononuclear Cell,PBMC),是指外周血中具有单个核的细胞,包括淋巴细胞(Lymphocyte)、单...
一、PBMC简介 外周血单个核细胞(Peripheral Blood Mononuclear Cell,PBMC),是指外周血中具有单个核的细胞,包括淋巴细胞(Lymphocyte)、单核细胞(Monocyte)、树突状细胞(DC)和其他少量细胞。PBMC是免疫系统的关键组成部分,在免疫学、传染病、恶性肿瘤、疫苗研发、移植治疗、个性化医学和毒理学等领域作为最基础的实验原料被...
Single cell spatial transcriptomic characterization using the CosMx Spatial Molecular Imager platform was conducted to analyze HIV-infected cells in formalin-fixed paraffin-embedded sections of splenic tissue surgically obtained from an HIV-infected humanized mouse model. Regulation of over a thousand human...
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can ha
Using single-cell RNA sequencing, we show that iNKT, MAIT and γδ T cells mature via shared, branched differentiation rather than linear maturation or TCR-mediated instruction. Simultaneous TCR clonotyping analysis reveals that thymic maturation of all three types is accompanied by clonal selection...
T-cell-proliferation-and-the-immune-response-Q2-2009 Product Information Dynabeads® Mouse T-Activator CD3/CD28 for activation of mouse T cells Frequently asked questions (FAQs) Mononuclear cells (MNC), also known as peripheral blood mononuclear cells (PBMC), are prepared from whole blood, buff...
(with a polyclonal sheep anti-rat IgG antibody covalently bound to the bead surface) and a monoclonal Antibody Mix, together designed for the depletion of CD8-negative leucocytes from mouse spleen or lymph node single-cell suspensions. Following the depletion procedure, the negatively isolated, ...
(MuSCs) from mouse. Lastly, we applied this protocol to a cryogenically preserved peripheral blood mononuclear cell (PBMC) sample from a patient (CLL2) whose chronic lymphocytic leukemia (CLL) had progressed on venetoclax treatment after a durable response. The sample was prepared together with ...
PBMC: Peripheral blood mononuclear cell PECO: Population, Exposure, Comparator, and Outcome PGD2: Prostaglandin D2 PRISMA: Preferred Reporting Items for Systematic Reviews and Meta-Analyses qPCR: Quantitative polymerase chain reaction RA: Rheumatoid arthritis RNA: Ribonucleic acid RNA-seq: RNA...
PBMC + CD34+HSC Humanized Models Model Types The humanized mouse model of the local human immune system is particularly effective for short-term or high-throughput research. Currently, we have the following model types available: Cancer TypeCell Lines ...