Human peripheral blood mononu- clear cells (PBMCs) were isolated from the buffy coat using a Ficoll density centrifugation, followed by CD14+ monocytes were separation using antibody coated microbeads (Miltenyi Biotec) as previously described36. To generate immature dendritic cells, approximately 106 ...
SCID-hu Thy/Liv mice were constructed and infected as previously described (Krowka et al., 1991, McCune et al., 1988, Su et al., 1995). Briefly, HIV-1 produced from PHA-activated PBMCs or mock supernatant was used to infect each graft. Approximately 2000 IU in 50 μl was used for...