In contrast, mitochondrial membrane potential (MMP) was higher in TE than ICM. Culture in ambient oxygen (20% O2) altered both aspects of mitochondrial function: the mtDNA copy number was upregulated in ICM, while MMP was diminished in TE. Embryos cultured in 20% O2 also exhibited...
Deposition of amyloid-β (Aβ) in the brain is one of the important histopathological features of Alzheimer’s disease (AD). Previously, we reported a correlation between cell adhesion molecule L1 (L1) expression and the occurrence of AD, but its relatio
Libraries were quantified by QuBit and BioAnalyzer DNA1000. All libraries were diluted to 10 nM and normalized by qPCR using the KAPA library quantification kit (KAPA; Cat no. KK4973). Libraries were pooled to equimolar concentration. Sequencing was performed with the Illumina Nextseq500 using...
structure and/or a slight dispersion of the chromosomes, with up to four scattered chromosomes; (iii) aberrant, characterized by a completely disorganized or abnormal or absent mitotic spindle and/or a total DNA disorganization on the MII plate or by the presence of noncondensed chromosomes (...
Tdtomato was amplified with the primers Rosa-F and Rosa-R, while normal controls could not amplify the 7265-bp DNA fragment. The 792-bp fragment was also a specific DNA fragment in RalARosa26-Tg/+ mice. (C) Immunoblot analyses of RalA were performed in RalARosa26-Tg/+ and RalA+/+ ...
DNA polymerase (Invitrogen) using thermocycler parameters set to 95 °C: 5 min (95 °C: 35 s, 52 °C: 30 s, 72 °C 40 s) × 45 cycles. Both reactions provided two products per reaction that were gel purified in a 2% agarose gel using a GeneJET Gel Extraction Kit (Thermo ...
(Vector Elite Kit, 1 µl/ml solution A and B in PB) for 11 min at 150 W under vacuum using the following cycle: 4 min on, 3 min off, 4 min on. The tissue was then rinsed in PB twice at 1 min each, at 150 W with the vacuum off and then exposed to DAB (0.5 µg/ml...
In eukaryotic cells, DNA double strand breaks (DSB) are primarily repaired by canonical non-homologous end joining (c-NHEJ), homologous recombination (HR) and alternative NHEJ (alt-NHEJ). Zinc finger and SCAN domain containing 4 (ZSCAN4), sporadically expressed in 1–5% mouse embryonic stem ...
To determine the optimal number of PCR cycles, a test PCR was performed using the KAPA library amplification kit (Kapa Biosystems, #KK2611). The final PCR product was run on an 8% TBE gel, and the 180–190 nt bands were selected. The PCR product was eluted overnight in DNA ...
Furthermore, ATP levels in response to repeated muscle contractions tended to improve, and mitochondrial activity was significantly enhanced in dystrophin supplemented-fibers. Conclusions Cell therapy-mediated dystrophin supplementation efficiently improved DMD muscle functions, as evaluated using newly developed...