人线粒体DNA拷贝数试剂盒|Human Mitochondrial DNA Copy Number Kit本DNA分析试剂盒用于通过比较实时PCR测定的线粒体(mt)和核(n)DNA,测定体内和体外人线粒体DNA拷贝数。 产品特点 该人线粒体DNA拷贝数试剂盒|Human Mitochondrial DNA Copy Number Kit产品特点参考产品说明书 ...
Human Mitochondrial DNA Copy Number Assay KitCatalog Number: MCN
The copy number of mitochondrial DNA per nuclear genome (i.e. per cell) was determined using real-time quantitative PCR (qPCR) according to Rooney JP76and Venegas V77protocol. The relative amount of mitochondrial genetRNA-Leu(UUR), to the nuclear single-copy gene β2-microglobulin (B2M)(mtD...
Total DNA was extracted from cell samples using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) with proteinase K and RNase treatment, according to the manufacturer's instructions. To quantify mtDNA copy number, real-time PCR was performed using a 72-well Rotorgene-3000 (Corbett...
with the accession number VC20220715. Genomic DNA was extracted using the Tiangen Biotech DNA kit (Beijing). For library construction, we utilized the NEBNext® library building kit with an insert size of 350 bp. The constructed DNA library was sequenced on the NovaSeq 6000 platform at Ben...
Although both ERAL1 and mitochondrial DNA/RNA can be released into the cytoplasm, the immunostimulatory effect of ERAL1 is indirect, since it can’t play the role as “signal 0” to initiate the immune response by interacting with PRR (Land, 2002). Therefore, ERAL1 can’t be considered a...
However, an increasing number of studies have reported that signals that promote cell death may not be fully activated despite the presence of DNA damage, in part because the signaling molecules that promote cell death are mutated or their expression is downregulated and, in part, because the ...
PolgA is DNA methylated at exon 2.12 Furthermore, in non-transformed mouse cells, DNA methylation negatively correlates with mtDNA copy number in a tissue-specific manner.9 This indicates that mtDNA copy number is regulated by DNA methylation of a mammalian nuclear-encoded gene and not of the mi...
Mutations in mitochondrial DNA (mtDNA) are responsible for several severe diseases that have no available cures. The multicopy nature of the mitochondrial genome means that mutations often exist in a state known as heteroplasmy, where both mutant and wil
officinalis was deposited at the Institute of Medicinal Plant Development and assigned the accession number so-01-yhy. The S. officinalis leaves were kept at −80 °C until use. For DNA sequencing, a plant genomic DNA kit (Tiangen Biotech, Beijing, Co., Ltd., Bejing, China) and an ...