Variations in mouse mitochondrial DNA copy number from fertilization to birth are associated with oxidative stress[J] . Catherine EM Aiken,Tereza Cindrova-Davies,Martin H Johnson.Reproductive BioMedicine Online . 2008 (6)Aiken, C.E., Cindrova-Davies, T. and Johnson, M.H. (2008) Variations ...
Mitochondria host key metabolic processes vital for cellular energy provision and are central to cell fate decisions. They are subjected to unique genetic control by both nuclear DNA and their own multi-copy genome - mitochondrial DNA (mtDNA). Mutations
The objective of this study was to investigate the molecular control of mitochondrial transcription and mitochondrial DNA (mtDNA) replication in mouse preimplantation embryos. We estimated the mtDNA copy number and characterized the expression patterns of two mitochondrial genes and several nuclear genes ...
PCR validation in removal of mitochondrial DNA and linear DNA from eccDNA To validate removal of mitochondrial DNA and linear DNA, 50 ng each of crude eccDNA and eccDNA purified, with the same amount of gDNA as a positive control, was used to amplify targeted regions of the mouse gene Actb...
Mammalian cI is composed of 44 different proteins, 7 of which are encoded by mitochondrial DNA, the other 37 being encoded by the nuclear DNA. Fourteen subunits, conserved from prokaryotes to humans, are directly involved in the redox and proton translocating enzymatic activity, whereas 30 ...
TGF-β1 did not increase cell number or mitochondrial DNA copy number but did increase mitochondrial membrane potential (MMP), which could explain the OCR increase. Reactive oxygen species (ROS) increased by 32% after TGF-β1 exposure for 48 h. TGF-β activated the mammalian target of ...
Hecht NB , Liem H , Kleene KC , Distel RJ , Ho SM ( 1984 ) Maternal inheritance of the mouse mitochondrial genome is not mediated by a loss or gross alteration of the paternal mitochondrial DNA or by methylation of the oocyte mitochondrial DNA . Developmental Biology , 102 , 452 – 461...
Yonekawa H, Moriwaki K, Gotoh O, Watanabe J, Hayashi J-I, Miyashita N, Petras ML, Tagashira Y (1980) Relationship between laboratory mice and the subspecies Mus musculus domesticus based on restriction endonuclease cleavage patterns of mitochondrial DNA. Japan J Genet 55:289–296. https://doi...
The inability to construct animal models of diseases caused by mutated mitochondrial DNA (mtDNA) has been a major obstacle to investigating pathogenetic mechanisms associated with specific mtDNA mutations. carrying a large-scale deletion in mtDNA have now been produced. They display some of the key ...
(ATAC-Seq), a method that tests genome-wide chromatin accessibility. Briefly, ATAC-seq allows the analysis of chromatin accessibility by sequencing DNA fragments that are bound by a hyperactive Tn5 transposase, which preferentially inserts sequencing adapters into open chromatin regions42. In the air...