Single-cell RNA-SeqDevelopmental trajectoryCholangiocyteFate decisionThe differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking. We employed ...
Bulk cell isolation and RNA preparation For bulk cell RNAseq, single-cell suspensions of day 5 thymi of BALB/cByJ mice were stained with anti-TCRγδ (GL3) and other surface markers and sorted using a Moflo-XDP (Beckman Coulter). TRIzol (Life Technologies)-chloroform (Sigma) extraction ...
The mechanisms of radiation-induced liver injury (RILI) has not been fully elucidated so far. In the present study, a RILI mouse model was constructed by exposing the liver to a single dose of 30 Gy X-rays. Liver injuries c
Here, we used spatial transcriptomics in combination with single-cell ATAC-seq and RNA-seq, lipidomics and functional assays to address how cells in the male murine liver are affected by age-related changes in the microenvironment. Integration of the datasets revealed zonation-specific and age-...
Single-cell RNA sequencing deciphers the mechanism of sepsis-induced liver injury and the therapeutic effects of artesunate. Liver samples were collected for preparing single-cell RNA transcriptome sequencing (scRNA-seq). The scRNA-seq analysis revealed that sepsis-induced a ... XL He,JY Chen,YL...
RNA-Seq has been revolutionizing the transcriptome study as it can effectively capture the whole transcriptome of various cell types under different conditions. Here we predicted 308 novel mouse embryonic lncRNAs from the RNA-Seq data of WT and Klf1 KO samples using a computational pipeline. The ...
Laboratory mouse strains have mosaic genomes derived from at least three major subspecies that are distributed in Eurasia. Here, we describe genomic variat
Single cell RNA-seq Dental germ development Spatiotemporal pattern Odontogenesis CD24+ odontogenic cells Plac8+ odontogenic cells 1. Introduction All epidermal organs, including teeth, hair follicles, mammary glands, and whiskers, develop from epithelial-mesenchymal interactions [1], [2], [3], [4...
we allocated the combinatorial expression of a curated marker gene list that is used to define different domains of progenitors in single-cell RNA-seq45to the spinal cord spots, including dorsal gene sets (D) by combinatorial marker expression of roof plate (RP) and dp1–dp6, medial gene set...
Here we present a compendium of single-cell transcriptomic data from the model organism Mus musculus that comprises more than 100,000 cells from 20 organs and tissues. These data represent a new resource for cell biology, reveal gene expression in poorly