Visual information-integration categories were constructed from a 2D stimulus space of orientations and spatial frequencies, in which the category boundary was determined by a 45° diagonal line6,53,58,85. In experiments with freely moving mice, the category space consisted of stationary square-wave...
因为前面介绍的都是斑马鱼中的crisper cas9谱系示踪方法,这里再来打卡一篇在小鼠中应用crisper cas9技术的文章,题目是An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells。知乎的标题因为有字数的要求,我这里做了修改,希望不要引起歧义。
Cell Cycle 12, 2355–2365 (2013). CAS PubMed PubMed Central Google Scholar Koyama, Y. et al. Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein. J. J. Pharmacol. 83, 351–354 (2000). CAS Google Scholar Yenari, M. A. & Giffard...
e (lLeft: the maternal genes show high expression levels in oocyte samples and are largely down-regulated in normal two-cell embryos. Some maternal genes are also still highly expressed in α-Amanitine treated two-cell embryos. (rRight: the zygotic genes are highly expressed in normal two-...
To explore the relationship between CSF-1 and ERS in Leydig cells, we treated Leydig tumor cell line with an activator Tuniamycin and an inhibitor 4-Phenylbutyrate of ERS. Our data showed that the CSF-1 expression in mouse Leydig cell lines decreased six-fold while reversely increasing five-...
Rhox6 facilitates the specification of PGCLCs by increasing the expression of Nanos3 To better investigate the mechanism by which Rhox6 induces PGCLC fate decisions, we constructed a Flag-tagged Rhox6-inducible cell line with a genome nonintegrated system (i-Rhox6), and found that short-term...
背景说明:C-X-C motif chemokine 2 (CXCL2,MIP-2) belongs to the intercrine alpha (chemokine CxC) family. It was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The expression of mouse MIP-2 is stimulated by ...
Drd2-Cremouse line labels type II afferents in the mid-basal region of the cochlea Full size image Full size image Comparison of expression between different molecular markers for type II afferents Full size image Full size image Data Availability ...
but also strategically occupies those that are silenced, consistent with a role of BMP4 in both activating and suppressing gene expression during PNT at the chromatin level. Our results reveal a previously unknown function of BMP4 in regulating nuclear architecture and link its targets ZBTB7A/B to...
Ikaros targets accounted for one-half of all genes up-regulated during B-cell lineage specification in vivo, explaining the essential role of Ikaros in this process. Expression of the Ikaros paralogs Ikzf1 and Ikzf3 increases incrementally during B-cell progenitor differentiation, and, remarkably, ...