因为前面介绍的都是斑马鱼中的crisper cas9谱系示踪方法,这里再来打卡一篇在小鼠中应用crisper cas9技术的文章,题目是An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells。知乎的标题因为有字数的要求,我这里做了修改,希望不要引起歧义。
c, d, Diversity score correlation with the number of genes expressed per tissue (c) or tissue cell-type (d). The red line corresponds to the linear regression curve. e, Trajectory analysis for a brain myeloid microglia cell. f, Heat map showing differential gene expression analysis of ...
Barletta, R. G., Kim, D. D., Snapper, S. B., Bloom, B. R. & Jacobs, W. R. Jr. Identification of expression signals of the mycobacteriophages Bxb 1, L1 and TM4 using the Escherichia-Mycobacterium shuttle plasmids pYUB75 and pYUB76 designed to create translational fusions to the la...
Consistent with previous findings, we observed that the protective action of l-serine against DMNQ-caused ROS elevation and consequent mitochondrial apoptosis in HT22 cells was well-reflected by changes in MFN1/2 and OPA1 expression and DRP1 phosphorylation, which were presumed to indicate restoratio...
Large-scale quantitative analysis of transcriptional co-expression has been used to dissect regulatory networks and to predict the functions of new genes discovered by genome sequencing in model organisms such as yeast. Although the idea that tissue-spec
“knocked into” the Drd1a gene locus downstream of a floxed NEOSTOP cassette [5]. Cell death follows Cre expression and Drd1a promoter driven tox-176 production at 1–2 weeks in the global line [3,6] and 5 weeks in the striatal line [4]. The Emx-1 promoter drives Cre expression ...
In contrast to 16-cell, in 32-cell embryos the outer and inner cells are equivalent to polar and apolar cells, respectively, as we confirmed by correlating the expression of pERM with the position of cells (n = 41; data not shown). In control 32-cell embryos (n = 23), ratio 20:12...
(RA) due to its enhanced expression in RA-derived bone marrow stromal cell lines. CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol ...
6c, f). Elevated Kv3.1 expression is a key differentiator between Pvalb and other inhibitory cell types, i.e., increased Kv3.1 results in a shorter spike width and fast afterhyperpolarization24,38,39,40. In terms of cellular morphology, pairwise comparison of morphology features (bifurcation ...
The simplest definition of cis-eQTLs versus trans, refers to genetic variants that affect expression in an allele specific manner, with implications on underlying mechanism. Yet, due to technical limitations of expression microarrays, the vast majority o