Single-cell whole-genome sequencing methods have undergone great improvements over the past decade. However, allele dropout, which means the inability to detect both alleles simultaneously in an individual diploid cell, largely restricts the application
in E14 cell line-derived EBs at day 14. Left bottom, Western blot analysis showing STRAP expression in inducible STRAP knockdown EBs with ß-Actin as a loading control. For (i, m), and (o), PSI values are shown below gel pictures. Experiments were repeated three independent times with ...
Mouse embryonic stem cells (mESCs) not only retain the property of self-renewal but also have the ability to develop into primordial germ cell-like cells (PGCLCs). However, knowledge about the mechanisms of transcriptional regulation is still limited. Rh
it might reflect the biology of the intestinal epithelium. In this context, it is interesting that a previous study found that changes in gene expression during intestinal cell maturation do not involve dramatic changes in chromatin accessibility [67]. ...
背景说明:C-X-C motif chemokine 2 (CXCL2,MIP-2) belongs to the intercrine alpha (chemokine CxC) family. It was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The expression of mouse MIP-2 is stimulated by ...
1 μL of plasma lysed in lysis buffer and total cell lysates from mouse fibroblast cell line L929 and human patient-derived glioblastoma cells served as positive controls. A challenging point of this protocol was to analyze EVs from individual mice with a limited volume of collected plasma. In...
(Fig.5c) would lead to an increased on-target base editing while maintaining the same precision levels. To this end, we used a docking site in the genome of the NIH/3T3 mouse cell line for FRT recombinase-assisted insertion of the T2A-linked and/or HHR-containingNd3,Cytb, CoIIandAtp6...
Accession codes:Single-cell genome sequences have been deposited in the European Nucleotide Archive (ENA) database under accession codePRJEB12768(ERP014275). How to cite this article:Bolton, H.et al. Mouse model of chromosome mosaicism reveals lineage-specific depletion of aneuploid cells and norm...
A comprehensive analysis for tooth regenerative medicine is required to investigate the cell heterogeneity and gene expression/regulation dynamics in incisors and molars before birth. Therefore, we proposed to create a cell atlas of the whole tooth germ in varied development stages in single cell ...
Each gene in this database is associated with one class label, which is either one of the three cell-types or a negative class label when it does not belong to any of the three classes. To discriminate genes with different class labels, we designed six classification tasks by constructing ...