(base) [bio-wenzl@login04 ~]$ export PATH=/work/bio-wenzl/cellranger-7.1.0/:$PATH 再次输入cellranger,既可以看到可以正常运行了: (base) [bio-wenzl@login04 ~]$ cellranger cellranger cellranger-7.1.0 Process 10x Genomics Gene Expression, Feature Barcode, and Immune Profiling data USAGE: ...
16 non-cancerous cell lines, and 8 uncategorized cell lines in which the disease types have not yet been classified (marked as “Unknown”; see Supplementary Data1)8,13. The CCLE 2019 cell line database6provides the transcriptomics data of a total of 1,019 cell...
流程: 1. log : NormalizeData 2. 找特征 : FindVariableFeatures 3. 标准化 : ScaleData 4. pca : RunPCA 5. 构建图 : FindNeighbors 6. 聚类 : FindClusters 7. tsne /umap : RunTSNE RunUMAP 8. 差异基因 : FindAllMarkers / FindMarkers VlnPlot(raw_sce, features = c("percent.rp", "percen...
cell cycle and gene expression regulation, in 1986, the first therapeutic protein—recombinant tissue‐type plasminogen activator (tPA, Activase; Genentech, San Francisco, CA, USA)—was obtained in the culture of immortalized Chinese hamster (Cricetulus griseus) ovary (CHO) cell line [129, 130]....
database (Unibind46), ChIP-seq (Cus_ChIP) and regulator perturbation (Cus_KO) experiments in the GM12878 lymphoblastoid cell line from Cusanovich et al.47and the intersection of ChIP and perturbation studies (Cus_KO+Cus_ChIP, Cus_KO+Unibind). In total, we had five gold standard networks....
其中以多囊卵巢综合征(Polycystic ovary syndrome, PCOS)尤为典型，是育龄期女性最常见的生殖内分泌代谢性疾病，其全球患病率高达11-13%【1】。PCOS不仅导致月经失调、排卵障碍和不孕，还与糖脂代谢紊乱密切关联【2】。更令人担忧的是，PCOS具有高度遗传性，PCOS患者的女儿患病风险是正常女性女儿的5倍，现有动物实验更...
In a neuroblastoma cell line, increased expression of P-gp and MRP1 was detected, and after administration of low-intensity pulsed ultrasound, the expression of P-gp was significantly decreased (Sun, Chen, et al., 2013; Sun, Li, et al., 2013). Enhanced BCRP expression has also been ...
其中以多囊卵巢综合征(Polycystic ovary syndrome,PCOS)尤为典型,是育龄期女性最常见的生殖内分泌代谢性疾病,其全球患病率高达11-13%【1】。PCOS不仅导致月经失调、排卵障碍和不孕,还与糖脂代谢紊乱密切关联【2】。更令人担忧的是,PCOS具...
GDSC gene-level mutation and methylation data (numbers in red) were prepared from raw data as part of the development of CellMinerCDB. Asterisks indicate molecular data under development, but not publicly available. Protein expression was determined by reverse-phase protein array. (C) Cell line ...
when all patients in the database were taking branded GA only (Supplementary Table 5;Supplementary Figure 2). To compare GA with Probioglat and further elucidate GA’s MOA, we measured gene-expression profiles induced by each in the THP-1 human monocyte cell line, using a genome-wide microar...