6.采用STR分型技术鉴定小鼠细胞时，如何判定细胞株存在交叉污染？答：小鼠为二倍体，因此正常小鼠细胞的STR位点峰图，或为单峰（纯合子）或双峰（杂合子）。如果分型图谱中，多个位点同时出现多等位基因（一般为3峰或4峰）时，则考虑细胞株存在交叉污染。7. 准备小鼠细胞的样本时，有哪些要求？答：培养中的细胞...
格式如下:The cell lines were characterized byGenetic Testing Biotechnology Corporation (Suzhou,China)using short tandem repeat (STR) markers. Selected high quality publications using our cell line authentication
苏州鉴达(Genetic Testing Biotechnology, GTB)采用STR分型技术,针对人源细胞23个基因座、小鼠源细胞19个基因座(18个小鼠源基因座+1个人源基因座TH01)进行检测,3130XL型遗传分析仪进行毛细管电泳,与DSMZ、Cellosaurus、ATCC等数据库进行比对,出具权威英文报告,为全国近400家科研机构(高校、研究所、医院、公司)提供高...
Selected high quality publications using our cell line authentication
英文名称 Mouse Original B Cell Line; BAF3 产品编号 CLM0016-RT 英文名称代码 BAF3 细胞培养条件 1640+10FBS+10ng/ml IL-3(鼠源)+1%P/S 价格说明 价格:商品在爱采购的展示标价,具体的成交价格可能因商品参加活动等情况发生变化,也可能随着购买数量不同或所选规格不同而发生变化,如用户与商家线下达成...
Cyagen specializes in generating knockout/transgenic mice, rats and cell line models, as well as virus packaging, vector cloning, stem cells and cell culture reagents.
(4)观察:每日观察细胞生长状态(培养基颜色、形态与密度等)并拍照。 传代 细胞密度达80%-90%开始传代 (1) 清洗:无菌下吸出培养基,用37℃预热的PBS清洗一次; (2) 传代培养:该细胞传代时不需要用胰酶消化。传代时,吸走部分培养液,留下少许培养液(例如使用T75培养瓶则留下10mL),用无菌细胞刮刮拭培养表面将细...
The induced pluripotent stem cell (iPSC) cell line Ctr8.2 (UEF-2B) was obtained from Virtanen Institute for Molecular Sciences-University of Eastern Finland67. Another iPSC line purchased from Gibco was also used for the functional assays (Seahorse measurements). The iPSC colonies were cultured an...
Creative Biolabs provides a wide array of human tumor cell lines for your anti-tumor drug discovery, including gastric, lung, liver, glioma etc.
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