Here, we report the application of single-nucleus RNA-seq on midbrain, striatum, and cerebellum of the 伪-syn-A53T mouse, a well-established PD mouse model, and matched controls, generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual...
Mammalian brain cells show remarkable diversity in gene expression, anatomy and function, yet the regulatory DNA landscape underlying this extensive heterogeneity is poorly understood. Here we carry out a comprehensive assessment of the epigenomes of mouse brain cell types by applying single-nucleus DNA...
While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border regions and combining single-cell RNA-sequencing with high-dimensional cytome
we generated a comprehensive cell atlas of more than 5,000 transcriptionally distinct cell clusters, belonging to more than 300 major cell types, in the whole mouse brain with high molecular and spatial resolution. Registration of this atlas to the mouse brain common coordinate framework allowed sys...
single-cell transcriptomics method, to generate a comprehensive, molecularly defined and spatially resolved cell atlas of the entire adult mouse brain. We selected a panel of more than 1,100 genes (Supplementary Table1) for MERFISH imaging. These genes were selected based on a whole-brain scRNA-...
During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and ce
Creation of the mouse brain cell-type atlas As part of the BICCN, we set out to build a comprehensive, high-resolution transcriptomic cell-type atlas for the entire adult mouse brain. We systematically generated two types of large-scale, single-cell-resolution transcriptomic datasets for all mou...
Using single-cell transcriptomic data, we assessed cell-type-specific manifestations of different hallmarks of ageing—such as senescence3, genomic instability4 and changes in the immune system2. This transcriptomic atlas—which we denote Tabula Muris Senis, or ‘Mouse Ageing Cell Atlas’—provides ...
Cell 169, 1276–1290 (2017). CAS PubMed Google Scholar Van Hove, H. et al. A single-cell atlas of mouse brain macrophages reveals unique transcriptional identities shaped by ontogeny and tissue environment. Nat. Neurosci. 22, 1021–1035 (2019). PubMed Google Scholar Da Mesquita, S. ...
Creation of the mouse brain cell-type atlas As part of the BICCN, we set out to build a comprehensive, high-resolution transcriptomic cell-type atlas for the entire adult mouse brain. We systematically generated two types of large-scale, single-cell-resolution transcriptomic datasets for all mou...