题目是:Comprehensive spatiotemporal mapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding。也是今天BioArt推送的公众号文章,因为加了spatiotemporal的关键词,感觉文章还不错呢。 Title 1.Abstract A fundamental interest in developmental neuroscience lies in the ability to map the ...
Recent advances in single-cell technologies have led to the discovery of thousands of brain cell types; however, our understanding of the gene regulatory programs in these cell types is far from complete1,2,3,4. Here we report a comprehensive atlas of candidatecis-regulatory DNA elements (cCRE...
Mammalian brain cells show remarkable diversity in gene expression, anatomy and function, yet the regulatory DNA landscape underlying this extensive heterogeneity is poorly understood. Here we carry out a comprehensive assessment of the epigenomes of mouse brain cell types by applying single-nucleus DNA...
During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and ce
Here, we report the application of single-nucleus RNA-seq on midbrain, striatum, and cerebellum of the 伪-syn-A53T mouse, a well-established PD mouse model, and matched controls, generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual...
While the roles of parenchymal microglia in brain homeostasis and disease are fairly clear, other brain-resident myeloid cells remain less well understood. By dissecting border regions and combining single-cell RNA-sequencing with high-dimensional cytome
(cCREs), most of which were found in the brain cell types. We therefore analyzed gene expression and the heterochromatin mark H3K9 tri-methylation (H3K9me3) in the mouse brain tissues using both single nucleus RNA-seq and Paired-Tag, a single cell multi-omics assay designed to profile both...
Single-cell technologies offer an unbiased assessment of the heterogeneity, dynamics and functions of immune cells. Here we provide a protocol that outlines all the steps involved in performing single-cell multi-omic analysis of the brain immune compartment. This includes a step-by-step description ...
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes, developmental time points and species. For 72% of genes, full-length isofo
细胞的培养采用公司专利产品小鼠嗅鞘细胞培养试剂盒(MouseBrainPrimaCell:NormalOlfactoryBulbEnsheathingCellsCatNo.3-4456)来优化目的细胞生长条件,以降低杂细胞(如脂肪细胞、纤维细胞等)的污染,同时保证质量的稳定。在公司技术部标准的操作流程下,小鼠嗅鞘细胞传3代仍可以保持原代细胞的分化状态,进而可以用于评估体外药物...