Recent insight has shown that the best predictor of protein-coding gene retention in the mitochondrion is hydrophobicity and/or how central a protein is to a given multiprotein complex [19,20,23,69]. These empirical predictors help us shed light on the explanations for retention. However, do ...
Complete mitogenomes ofNephila pilipes,Trichonephila antipodiana,T. vitiana(previouslyN. vitiana) andT. clavatawith BRIG visualization showing the protein-coding genes, rRNAs and tRNAs. GC skew is shown on the outer surface of the ring whereas GC content is shown on the inner surface. The an...
Cells were starved before the CRC measurements in a 2.5 mM (LG) glucose-containing medium for 24 h. Histograms represent nmols of Ca2+ per µg of protein that are necessary to cause PTP opening. Data are mean (expressed as % of controls) ± SEM of three experiments, *p =...
Equal protein amounts were loaded and confirmed with succinate dehydrogenase complex, subunit A (SDHA) staining. Data represent mean ± SEM values derived from four independent determinations. The statistical significance was determined using one-way ANOVA followed by Dunnett’s test *p < 0.05, when...
The arrow indicates transcription direction of the protein-coding genes. The genes encoding Complex I proteins are in yellow, those for Complex II are in pink, ATP synthase is in green, other protein coding genes are in purple, tRNAs are in blue, and rRNAs are in red. b Phylogenetic tree...
Protein concentration was subsequently measured using a BCA assay. All Seahorse values were normalized to the appropriate protein levels and quantified. For OCR calculations: basal respiration is the resting respiration minus rotenone/AntA titration; ATP-linked respiration is the basal respiration minus ...
mitochondrial sirtuin network identified five subnetworks containing known protein complexes, including mitochondrial ribosome large subunit (MRPL), complex I, mitochondrial inner membrane organization system (MitOS/MINOS), oxoglutarate dehydrogenase complex (OGDHC), and pyruvate dehygrogenase complex (PDHC)...
4e), implying that the insertion of mtDNA sequences to nDNA may affect the stability of nDNA thus leading to complex SVs. As expected, this trend was not found for simple SVs, meaning that the initiation of simple SVs was not affected by non-ref NUMTs (Fig. 4e)....
protein (Fig.1c). We also observed a loss of mtCU components MCUB and EMRE (Fig.1c), likely attributed to protease mediated degradation of the other structural/channel-forming mtCU components24. Voltage-dependent anion channel (VDAC) and the UQCRC2 (Ubiquinol-cytochrome-c reductase complex ...
TFAM is hydroxylated by EGLN3 and subsequently bound by the von Hippel–Lindau tumour-suppressor protein, which stabilizes TFAM by preventing mitochondrial proteolysis. Cells lacking wild-type VHL or in which EGLN3 is inactivated have reduced mitochondrial mass. Tumorigenic VHL variants leading to ...