miRNA-140-5pWnt1/β-catenin信号通路Runt相关转录因子2碱性磷酸酶目的 探讨miRNA-140-5p对人骨髓间充质干细胞(hBMSCs)成骨分化的影响及其可能的相关机制.方法 构建miRNA-140-5p模拟物(Mimic组),抑制剂(Inhibitor组),重组质粒pcDNA3.1-Wntl及其相应的阴性对照并转染hBMSCs,应用CCK-8,茜素红染色,Elisa,qRT-PCR,...
【摘要】目的:研究补肾活血方含药血清对白细胞介素-1β(IL-1β)刺激的人原代软 骨细胞 miRNA-140 及其靶基因表达的影响.方法:通过采用生理盐水和补肾活血方 分别灌胃雌性 SD 大鼠制备空白血清和含药血清.将人软骨细胞种植于 6 孔板并且 分为 3 组.空白对照组和模型对照组细胞培养的血清采用大鼠空白血清,含药...
目的:研究补肾活血方含药血清对白细胞介素-1β(IL-1β)刺激的人原代软骨细胞miRNA-140及其靶基因表达的影响.方法:通过采用生理盐水和补肾活血方分...
miRNA-140-5p软骨细胞目的:研究补肾活血方含药血清对白细胞介素-1β(IL-1β)刺激的人原代软骨细胞miRNA-1 40及其靶基因表达的影响.方法:通过采用生理盐水和补肾活血方分别灌胃雌性SD大鼠制备空白血清和含药血清.将人软骨细胞种植于6孔板并且分为3组.空白对照组和模型对照组细胞培养的血清采用大鼠空白血清,含药...
Aim: To discuss the effects and mechanisms of miRNA-140-5p in development of Parkinson's disease. Methods: The colon tissues which were from 30 PD patients and 30 healthy person were collected and measured miRNA-140-5p and TLR4 expression by ISH or IHC assay; and collecting the blood from...
Reversing the miRNA -5p/-3p stoichiometry reveals physiological roles and targets of miR-140 miRNAsdoi:10.1101/2021.10.12.463698Young CameronCaffrey MelissaChristopher JantonTatsuya KobayashiCold Spring Harbor Laboratory
Conclusion: miRNA-140-5p improved Parkinson's disease induced inflammation via directly TLR4.doi:10.1166/jbt.2018.1905Ye, MingChen, YuhuaXie, JingYu, MeilingShi, PengXu, WenfangGui, ZhonghaoLiu, XiaolinJournal of Biomaterials and Tissue Engineering...
microRNA‐140‐5p (miRNA‐140‐5p)myeloid differentiation protein 88 (MyD88)nuclear factor κB (NF‐κB)Shikonintoll‐like receptor 4 (TLR4)Shikonin is an anti﹊nflammatory agent extracted from natural herbs. The aim of this study is to explain the treatment effects and mechanism of Shikonin ...
Yang, Y.; Liu, D.; Xi, Y.; Li, J.; Liu, B.; Li, J. Upregulation of miRNA-140-5p inhibits inflammatory cytokines in acute lung injury through the MyD88/NF-κB signaling pathway by targeting TLR4. Exp. Ther. Med. 2018, 16, 3913-3920. [CrossRef] [PubMed]...
在肾母细胞瘤细胞株G401及SK-NEP-1中,发现过表达miRNA-140-5p可有效降低G401及SK-NEP-1的增殖率,将miRNA无关序列(miRNA-con)转染组细胞增殖率设为100%,与其相比,转染miRNA-140-5p的G401细胞组的增殖率为(87±15)%比(100±20)%,SK-NEP-1细胞组为(91±12)%比(100±9)%,组间比较,差异均有统...