microRNA(miRNA)是由20～23个核苷酸组成的单链非编码RNA。在生物体内miRNA可以与一个或者多个mRNA的3’UTR区互补,降解或抑制靶基因mRNA,从而抑制靶基因的表达[6]。 笔者采用高通量的miRNA array方法筛选了CFA炎性痛模型大鼠背根神经节(DRG)组织内的差异表达miRNA,结果发现miRNA-133b-3p在CFA大鼠DRG组织中表达下调...
miRNA-133b targets FGFR1 and presents multiple tumor suppressor activities in osteosarcomaOsteosarcoma, miR-133b, Fibroblast growth factor receptor 1, Tumor suppressor, Ras/MAPK signaling, PI3K/Akt signalingBackground Osteosarcoma (OS) is the most common bone malignancy prevalent in children and young ...
rno—miRNA-133b对大鼠骨髓间充质干细胞增殖的影响
Objective: To investigate the effect of miRNA-133b on proliferation and apoptosis of bone marrow derived mesenchymal stem cells(BMSCs),and preliminary analyze its possible target and mechanism.Methods: Primary cultured BMSCs of rats were transfected with rno-miRNA-133b mimic and inhibitor by siPORT...
目的:观察CFA致炎大鼠脊髓腰膨大组织内miRNA-133b-3p与P2X4受体表达的相关关系.方法:大鼠足底注射CFA后,0 h,2 h,1 d,3 d检测大鼠机械痛和热痛的痛阈值变化,且在这四个时间点取脊髓腰膨大组织,提取总RNA和蛋白质,检测miRNA-133b-3p表达量的变化,同时检测P2X4受体mRNA和蛋白的表达量的变化.结果:与对照组相比...
目的探讨微小RNA-133b(miRNA-133b)在胃癌组织中的表达情况及其对基质金属蛋白酶9(MMP-9)表达的影响.方法选取2015年12月至2018年1月于该院胃肠外科住院行胃癌根治手术的患者78例,留取胃癌组织及癌旁组织(距肿瘤边界6cm以上),实时荧光定量PCR(RT-qPCR)检测miRNA-133b与MMP-9mRNA在两组标本中的表达水平,免疫组织...
目的探讨microRNA-133b(miRNA-133b)在卵巢癌组织中的表达情况及对基质金属蛋白酶-9(MMP-9)表达的影响.方法选取2016年3月至2019年10月住院行手术治疗卵巢癌患者52例,留取卵巢癌组织标本(病例组),随机选取同一时期55例因子宫肌瘤或宫颈癌行全子宫双附件切除患者的正常卵巢组织为对照组,荧光定量PCR(RT-qPCR)检测...
Objective To investigate the mechanism of moxibustion in the treatment of diarrhea-predominant irritable bowel syndrome (IBS-D), by observing the effects of moxibustion at Tianshu (ST25) and Shangjuxu (ST37) on microRNA-133b (miRNA-133b), pituitary homeobox family factor 3 (Pitx3)/tyrosine ...
Modulation by cocaine of dopamine receptors through miRNA-133b in zebrafish embryos . PLoS One 7 :e52701. doi:.10.1371/journal.pone.0052701 [ Cross Ref ]Barreto-Valer K, Lopez-Bellido R, Macho Sanchez-Simon F, Rodriguez RE. Modulation by cocaine of dopamine receptors through miRNA-133b in...
miRNA microarray profilingDihydrotestosteronemiR-133bHuman dermal papilla cellsWnt/beta-cateninObjective: Androgenetic alopecia (AGA), a common alopecia, is often accompanied by abnormal expression of multiple miRNAs. This study aims to investigate abnormally expressed miRNAs in patients with AGA and their...