The functions of hsa-miR-34a included three knowledge domains: molecular function, biological process and cellular component. Two and three miR-34a target islands were observed in autophagy relative genes, beclin1 and sestrin 2 mRNA, respectively. Sequence analysis showed the insert sequence was corre...
GeneForward primer sequence (5'-3')Reverse primer sequence (5'-3') Runx25' AGA CCA GCA GCA CTC CAT AT 3'5' CTC ATC CAT TCT GCC GCT AGA 3' ALP5' ATG GCT CAC CTG CTT CAC G 3'5' TCA GAA CAG GGT GCG TAG G 3' OCN5' AGG GCA GTA AGG TGG TGA AT 3'5' GCA TTA ACC AAC...
by mir 34a D Matching sequences among mir 34a and BCL 2 3′UTR in different species Day(s) after transfection Day(s) after transfection A B M T T a s s a y ( O D 5 7 0 ) M T T a s s a y ( O D 5 7 0 ) 220 3蛳 4 期 mir-34a 在神经元中的表达特征及功能研究 ...
The porcine miR-34a was an intergenic miRNA, and its sequence was highly conserved among mammals. Subcellular localization analysis showed that miR-34a was distributed in both nucleus and cytoplasm in porcine GCs. Bioinformatic analysis revealed that the MRE of miR-34a was located at -194 nt - ...
MicroRNAs (miRs) are single stranded and evolutionarily conserved sequences of short non-coding RNAs (~21–25 nucleotides)7and act as endogenous repressors of gene expression by mRNA degradation and translational repression. They have been shown to have important roles in cell differentiation, develop...
Interestingly, in this study, we found that the target sequence of miR-34a to Sirt1 mRNA was in the overlapping region (Fig. 1A). To examine the expression patterns of Sirt1 AS lncRNA, Sirt1 mRNA and miR-34a, their levels were detected by qPCR or western blotting during myoblast ...
which in the context of liver cells may be associated with tissue remodeling and regeneration.72Hypomethylation of an alternative promoter region upstream from MIR34A coding sequence was observed in chronic lymphocytic leukemia compared to healthy B cells, which was accompanied by increased miR-34a expr...
The position of the predicted miR-34a-5p binding sites in the respective 3′UTR reporter constructs and additionally the sequences of the binding sites of miR-34a-5p in the different 3′UTRs as well as the mutated binding sites (underlined) are shown. a PLCG1-3′UTR reporter vector, b...
The miR-34a coding sequence differs from that of the other miR-34 family members. miR-34a is encoded by one transcript, while miR-34b and miR-34c are encoded by another transcript. All the members of the miR-34 family have been reported to be tumor suppressor miRNAs. The expression level...
. Primer sets sequences used for qPCR in this study. Figure S1. CpG sites in the promoter region of miR-34a (n=14), and sequences of the primers used for amplification of converted DNA for sequenom massarray analysis. Figure S2. Expression of some well-known targets of miR-34a in 563...