本发明属于生物医药领域,涉及一种miR‑199a‑3p作为诊断脂质代谢紊乱的分子标志物在高脂血症及其并发症治疗中的应用。实验结果显示,hsa‑miR‑199a‑3p不仅在LDLR‑/‑和APOE‑/‑小鼠中明显升高,在高脂血症患者同样显著升高,且其分子水平与受检者总胆固醇平呈正相关。根据miRNA‑199a‑3p的表达...
miR‑199a‑3p和miR‑199a‑5p在NSCLC中扮演着抑癌角色。另外,两种基因能增强NSCLC中EGFR‑T790M引起的吉非替尼药物敏感性。本发明在临床上为利用miRNA来诊断和治疗非小细胞肺癌,以及提供药物治疗靶点方面提供了一定的应用价值。权利要求书1页 说明书15页 附图7页CN 114642679 A2022.06.21CN 114642679 A ...
以上结果表明,在CAP信号肽的引导下,MSCsSC的工程外泌体显著提高了外泌体miR-199a-3p对软骨细胞的递送效率,并显示出软骨细胞自噬的增强,从而在OA细胞模型中具有治疗潜力。 8.在体内通过工程MSCsSC-Exos介导软骨靶向miR-199a-3p的...
miR-199a-3p 生物信息学 mTOR GO富集分析 KEGG Pathway分析 膀胱癌 PI3K-Akt MAPK 蛋白聚糖目的为深入研究miR-199a-3p在膀胱癌形成和发展中的作用提供理论依据。方法分析miR-199a-3p序列,预测其靶基因和转录因子,并对靶基因进行GO富集和KEGG Pathway分析;构建TF-miR-199a-3p-靶基因网络调控图。结果 miR-199a...
MicroRNAs (miRNA) have rapidly emerged as modulators of gene expression in cancer in which they may have great diagnostic and therapeutic import. MicroRNA-199a-3p (miR-199a-3p) is downregulated in several human malignancies including hepatocellular carcinoma (HCC). Here, we show that miR-199a-...
摘要：本发明属于临床检测领域，公开了一种辅助诊断神经母细胞瘤的PCR检测试剂盒及检测has‑miR‑199a‑3p(以下简称miR‑199a‑3p)表达量的方法。本发明的试剂盒包括外泌体冲洗液及外泌体RNA提取试剂、miR‑199a‑3p特异性引物、miR‑199a‑3p反转录试剂、以及实时荧光定量PCR检测试剂。本发明的PCR...
99a-3p); miR-590-3p MicroRNA-199a-3p (miR-199a-3p); miR-590-3pMicroRNA-199a-3p (miR-199a-3p); miR-590-3pRodent studies suggest exogenous human miR-590-3p and miR-199a-3p could help promote cardiac repair following MI.Nature Publishing Group USScience-Business eXchange...
miR-199a-3p was significantly downregulated in the majority of human hepatocellular carcinoma (HCC) tissues and HCC cell lines. Yes associated protein 1 (YAP1) was overexpressed in human HCC, which promoted HCC development and progression by upregulating Jagged1 and activating the Notch pathway. ...
miR-199a-3p ultrasound-targeted nanobubble destruction liposome nanobubbles Introduction Hepatocellular carcinoma (HCC) is a malignant tumour that seriously threatens human health, manifesting as pain, weight loss, ascites, jaundice and decreased liver function, which may lead to death in advanced stages...
本文首先运用RT-qPCR技术探究了miR-199a-3p在C2C12成肌细胞分化过程中的表达趋势;用miR-199a-3p的mimic和inhibitor转染C2C12成肌细胞并诱导分化后,免疫荧光检测超表达或干扰miR-199a-3p对肌管形成的影响,RT-qPCR和WB技术探究对成肌分化标记基因和其靶基因mRNA和蛋白表达的影响;然后构建了miR-199a-3p靶基因的荧光...