皮损中HIF-1α mRNA与银屑病患者PASI呈正相关,皮损中miR-199a水平与银屑病患者PASI呈正相关。进一步以PASI为因变量,以与因变量呈线性关系的HIF-1αmRNA和miR-199a为自变量,进行多元逐步回归分析,发现HIF-1αmRNA和miR-199a对PASI...
目的探讨miR-199a对高糖刺激人肾小管上皮细胞间质转化(EMT)的影响。方法体外培养人肾小管上皮细胞,按不同糖浓度培养分为低糖组(LG)、高渗组(HM)和高糖组(HG),按是否转染miR-199a抑制剂(miR-199ai)分为miR-199a抑制组(mi...
miR-199a抑制物增加SH-SY5Y细胞Sirt1蛋白表达;当SH-SY5Y细胞处于氧化损伤时,miR-199a模拟子可降低细胞超氧化物歧化酶活性和升高丙二醛含量,miR-199a抑制物增加细胞活力、降低细胞内超氧化物歧化酶含量、升高超氧化物歧化酶活性和降低丙二醛含量。
关键词: miR-199a;外泌体;间充质干细胞;心肌细胞 H9c2;缺糖缺氧/复糖复氧;线粒体;Akt/HIF-1α/DRP1 轴 中图分类号:R363 文献标志码:A 文章编号: 1674-6376(2024)10-2317-09 DOI:10.7501/j.issn.1674-6376.2024.10.011 MiR-199a-modified mesenchymal stem cell-derived exosomes promote mitochondrial ...
miR-199a调控IGF1表达对机械刺激下成骨细胞分化的影响 林维龙\吴晓沛2,王晓明何薇薇1 (1.河北北方学院附属第一医院 口腔科，河北张家口075000;2.张家口学院，河北张家口075000;3.河北北方学院，河北张家口075000)[摘要]目的：探讨微小RNA (m iR)-199a在机械牵张力刺激下MC3T3-E1细胞中的表达变化及其对牵张力刺激...
目的 探讨miR-199a修饰的间充质干细胞(MSCs)来源的外泌体修复缺糖缺氧/复糖复氧模型心肌细胞H9c2线粒体的作用机制。方法 体外培养 MSCs,转染 miR-199a mimics 或 miR-NC,48～72 h 后收集外泌体,实时荧光定量PCR(qRT-PCR)法检测外泌体的 miR-199a水平。将 H9c2细胞分
However, overexpression of both miR-199a and Hif1α reduced DDP-induced apoptosis in C13* cells. In conclusion, miR-199a may change DDP resistance in ovarian cancer by regulating Hif1α. 展开 关键词: miR-199a ovarian cancer cisplatin resistance Hif1α ...
It was previously demonstrated that microRNA-199a (miR-199a) was down-regulated in () partially caused by hypermethylation of its promoter. miR-199a is encoded by two loci in the genome, miR-199a-1 on (Chr) 19 and miR-199a-2 on Chr 1. Both loci encode the same miR-199a. Anothe...
It was previously demonstrated that microRNA-199a (miR-199a) was down-regulated in testicular germ cell tumor (TGCT) partially caused by hypermethylation of its promoter. miR-199a is encoded by two loci in the human genome, miR-199a-1 on chromosome (Chr) 19 and miR-199a-2 on Chr 1...