探讨miR-196a在非小细胞肺癌(NSCLC)组织及细胞系中的表达,以及抑制或过表达miR一196a对 NSCLC细胞增殖能力的影响及其作用的靶基因。方法 实时定量PCR(QPCR)技术检测NSCLC组织及细胞系中miR一196a的 表达水平,通过转染miR一196ainhibitors及miR.196amimics抑制或上调miR一196a的表达水平,并通过QPCR检测转染效率。
miR-196a调控p27^kip1促进非小细胞肺癌细胞增殖的机制研究
目的:探讨微小RNA-196a(miR-196a)在胰腺癌细胞系中表达状况及反义miR-196a对人胰腺癌PANC-1细胞生物学行为的影响.方法:miR-196a采用实时荧光定量PCR检测.人工化学合成反义miR-196a并转染 PANC-1细胞.CCK-8法检测细胞增殖.流式细胞术检测细胞凋亡情况.通过划痕损伤实验和Transwell小室细胞侵袭、迁移实验... 查看...
摘要: 目的:探讨微小RNA-196a(miR-196a)在胰腺癌细胞系中表达状况及反义miR-196a对人胰腺癌PANC-1细胞生物学行为的影响.方法:miR-196a采用实时荧光定量PCR检测.人工化学合成反义miR-196a并转染 PANC-1细胞.CCK-8法检测细胞增殖.流式细胞术检测细胞凋亡情况.通过划痕损伤实验和... 查看全部>> ...
PRDM16是miR-378a,miR-155,miR-199a,miR-382的共同靶基因;PGC-1α是miR-196a,miR-199a,miR-382的共同靶基因.结论(1)利拉鲁肽可显著降低高脂喂养C57BL/... 张盼盼 - 《江苏大学》 被引量: 0发表: 2021年 MicroRNA-133a-1 regulates inflammasome activation through uncoupling protein-2 miR-133a-...
miR-196a-5p 可作为预测胃癌顺铂耐药和生存不良的有效生物标志物。补骨脂素通过 miR-196a-5p/HOXB7/HER2 信号通路抑制细胞增殖和促进凋亡,增加胃癌细胞对顺铂的敏感性,从而逆转胃癌细胞对顺铂的耐药性。补骨脂素与顺铂联合应用可提高化疗效果,...
MiR-196a is upregulated in gastric cancer and promotes cell proliferation by downregulating p27(kip1). Y, Li SQ, Wang ZX, Cao XF, De W (2012) MiR-196a is upregulated in gastric cancer and promotes cell proliferation by downregulating p27(kip1). ... M Sun,XH Liu,JH Li,... - ...
miR-196a;MAP3K1;PI3K-Akt pathway;IMF;RNA-seq 1. Introduction Goat meat is widely recognized as a high-quality meat that fully meets the healthy eating habits of modern society. It is well received by consumers because of its tender meat, unique flavor, and delicious taste. Intramuscular fat...
Therefore, high miR-196b expression and low miR-378a-3p and miR-486-5p expression were indicated as useful biomarkers for prediction of the efficacy of vaccine treatment for patients with metastatic CRC. In a planned phase III study, expression levels of these 3 miRNAs (miR-196b-5p, miR-...
Correspondingly, the above PEITC-induced activation of the ROS-MiR-135a-Mitochondria dependent apoptosis pathways in malignant glioma was attenuated by pre-transfection with miR-135a inhibitor, pre-treatment with multidrug resistance-associated protein 1 (MRP1) inhibitor Sch B, or combination with ...