Human MIR103A2 qPCR Primer Pair,即人MIR103A2 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR...
In contrast, microRNA (miR)‐30c‐1‐3p/miR‐103a‐2‐5p expression was significantly downregulated in tumor tissues and cells. miR‐30c‐1‐3p/miR‐103a‐2‐5p overexpression could inhibit AR‐V7 expression, suppress VCaP cell growth, and inhibit AR‐V7 downstream factor expression by ...
检测NCM460正常结肠上皮细胞和4种结直肠癌细胞系中的miR-103a-3P及PFK-2 mRNA表达,严格按照RT-PCR试剂盒步骤进行检测,分别取待检测细胞,加入1 ml Trizol 试剂,匀浆器充分匀浆以提取总RNA,提取完成后用电泳仪检测其完整性,用cDNA 第一链合成试剂盒进行逆转录合成 cDNA,然后利用RT-PCR仪,在95 ℃条件下预变性5 ...
LncRNA LTSCCAT promotes tongue squamous cell carcinoma metastasis via targeting the miR-103a-2-5p/SMYD3/TWIST1 axis Mo Liu, Qingwen Liu, Song Fan, Feng Su, Chun Jiang, Guanming Cai, Youyuan Wang, Guiqing Liao, Xinyuan Lei, Weixiong Chen, Junming Bi, Weiqi Cheng, Luo...
最后得出结论,肝癌细胞分泌的外泌体miR-103通过靶向多种内皮连接蛋白来增加血管通透性并促进肿瘤转移,分泌的miR-103可作为潜在的治疗靶标和HCC转移的预测标记。 实验思路上很简单,首先在肝细胞癌病人血清和肝癌细胞培养上清中检测到了miR-10...
目的 探讨miR-181a调控PTEN诱导激酶1(PINK1)/帕金森病相关基因(Parkin)通路对骨质疏松(OP)大鼠破骨细胞线粒体自噬的影响.方法 健康雌性SD大鼠20只,随机分为OP模型组(n=10)与对照组(n=10).OP模型组大鼠制备OP模型,提取OP大鼠破骨细胞,设置OP组(未转染),si-miR-181a组(转染si-miR-181a载体质粒),si-NC组...
miR-103a-2-5pmiR-30c-1-3pproliferationprostatecancerAndrogens and androgen receptors are vital factors involved in prostate cancer progression, and androgen ablation therapies are commonly used to treat advanced prostate cancer. However, the acquisition of androgen ablation therapy resistance remains a ...
In addition, overexpressed miR-103a-2-5p reduced both the relative expression of Nrf2/HO-1 pathway-related proteins and the ratio of GSH/ROS, leading to the excessive intracellular ROS that may promote AML cell apoptosis. In the mouse model, the delivery of miR-103a-2-5p through CLPs ...
miR-103a荧光实时定量PCR目的 探讨miR-103作为非侵袭性生物学标志物对非酒精性脂肪性肝病(NAFLD)代谢异常的筛查意义.方法 以健康人群和NAFLD患者为研究对象,采集血清样本进行生化指标检测.采用Nucleo ZOL法分离血清miRNA,SYBR Green法对miR-103a-2进行相对定量分析.Speraman法对miR-103a-2和生化指标进行相关性分析....
Tumor-derived exosomal miR-103a-2-5p facilitates esophageal squamous cell carcinoma cell proliferation and migrationGAO, D.-C.HOU, B.ZHOU, D.LIU, Q.-X.ZHANG, K.LU, X.ZHANG, J.ZHENG, H.DAI, J.-G.European Review for Medical & Pharmacological Sciences...