A Bioinformatics database was used to identify Parkin-targeting microRNAs (miRNAs). Then, miR-103a-3p agomir, miR-103a-3p antagomir and Parkin siRNA were used to assess the effects of miR-103a-3p/Parkin/Ambra1 signaling-mediated mitophagy in PD in vitro and in vivo. The protein and ...
与PI组相比,MP组细胞增殖及葡萄糖摄取水平,乳酸含量明显降低(P<0.05).双荧光素酶报告结果显示,转染miR-103a-3P后可显著促进PFK-2-3′-UTR-WT的荧光素酶活性(P<0.05),但对突变基因无显著影响(P>0.05),且PFK-2与miR-103a-3P呈正相关.结论抑制miR-103a-3P表达可降低结直肠癌细胞增殖,并有效改善糖酵解,...
miR-103a-3p agomir could alleviate liver injury including the tissue injury and mitochondrial damage, inhibit the secretion of inflammatory factors, and decrease the apoptosis of liver cells. The high-mobility group B1 (HMGB1) was overregulated in sepsis, and it was a downstream target gene of ...
目的探讨miR-103a-3p在先天性巨结肠(Hirschsprung disease,HSCR)发生,发展中的作用及机制.方法RT-PCR检测miR-103a-3p在HSCR狭窄段及扩张段结肠肠管组织标本中的表达差异.CCK-8和Transwell法评估miR-103a-3p对细胞增殖和迁移的影响.运用KEGG,GO以及蛋白质-蛋白质相互作用网络分析预测潜在靶基因.通过RT-PCR,自动免...
肝脏浸润、淋巴结转移、SIX4高表达是胆囊癌患者预后的危险因素（P 均<0.05），miR -103a -3p 高表达是保护因素（P <0.05）。结论 胆囊癌组织中miR -103a -3p 表达上调，SIX4表达下调，且与胆囊癌恶性临床病理特征和生存率低下有关。关键词：胆囊癌；微小RNA -103a -3p ；SIX 同源盒蛋白4；病理特征 ...
Currently, for the 5′ untranslated region (5′ UTR) of mRNAs, very few naturally occurring examples exist whereby the targeting miRNA down-regulates the expression of the corresponding mRNA in a seed-dependent manner. Here we describe and characterize two miR-103a-3p target sites in the 5′...
症组血清miR-129-5p,miR-103a-3p水平明显降低,TNF-α,IL-6,HMGB1水平明显升高,差异均有统计学意义(P<0.05).死亡组血清miR-129-5p,miR-103a-3p水平明显低于生存组,TNF-α,IL-6,HMGB1水平明显高于生存组,差异均有统计学意义(P<0.05).多因素Logistic回归分析结果显示,miR-129-5p,miR-103a-3p水平升高是...
Mechanistically, the rs884225 C allele enhanced EGFR expression by altering the miR-103a-3p binding site, thus impairing miR-103a-3p鈥檚 anti-tumourigenic function. As a tumour suppressor gene, miR-103a-3p expression correlated with overall and recurrence-free survival in NSCLC patients. ...
摘要: 目的 探讨血清hsa-miR-22-3p、hsa-miR-103a-3p水平检测在诊断子宫腺肌病中的应用.方法 将2017年2月至2018年2月该院收治的子宫腺肌病患者216例纳入观察组,同时招募同时间于该院体检的60名健康者作对照组.采用实时荧光定量PCR(qRT-PCR)检测所有受试者血清中hsa-miR-22-3... 查看全部>> ...
C-erbB-2、miR-103a-3p、ANXA7在子宫内膜癌组织中的表达及相关性研究 中国性科学 ２０２１年１２月 第３０卷 第１２期 ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＨｕｍａｎＳｅｘｕａｌｉｔｙ， Ｄｅｃｅｍｂｅｒ２０２１， Ｖｏｌ．３０，Ｎｏ．１２ ·７９ ·床疗效观察［Ｊ］．广州中医药大学学报，２０１９，...