miR-939-5p诱导型一氧化氮合酶人视网膜微血管内皮细胞目的:探究mi R-939-5p对糖尿病性视网膜病变和人视网膜微血管内皮细胞(HRMEC)的调控作用.方法:将mi R-939-5p模拟物(mi R-939-5p-mimic)或mi R-939-5p抑制剂(mi R-939-5p-inhibitor)转染到HRMEC中,并将细胞用高糖(HG组,25 m M)或低糖(LG组,5 ...
miR-939-5p directly targeted ARHGAP4. Either miR-939-5p down-regulation or ARHGAP4 overexpression inhibited viability, invasion and migration of pancreatic cancer cells. However, ARHGAP4 overexpression markedly inhibited the increased viability, migra- tion, and invasion induced by miR-939-5p up-...
Either miR-939-5p down-regulation or ARHGAP4 overexpression inhibited viability, invasion and migration of pancreatic cancer cells. However, ARHGAP4 overexpression markedly inhibited the increased viability, migration, and invasion induced by miR-939-5p up-regulation in pancreatic cancer cells. Conclusion...
受microRNAs(MiRNAs)影响的主要应激反应是衰老,这是一种由亚致死性细胞损伤引发的无限期生长停滞状态。在这里,通过生物信息学分析和实验验证,作者确定miR-340-5p是一个新的引起细胞衰老的miRNA。MiR-340-5p在不同的衰老模型中高度表达,m...
(对照组).qPCR检测miR-939-5p的转染效率.Transwell迁移实验和四甲基偶氮唑蓝比色(MTT)增殖实验检测miR-939-5p对肝癌细胞迁移和增殖能力的变化.生物信息学软件预测miR-939-5p的靶基因.双荧光素酶报告基因验证miR-939-5p与靶基因的相互作用.qPCR和Western blotting检测高表达miR-939-5p后靶基因在mRNA和蛋白水平的...
目的 探讨胰腺癌组织中微小RNA-939-5p(miR-939-5p)与Rho GTPase激活蛋白4(ARHGAP4)的表达变化及临床意义.方法 用荧光定量PCR技术检测98例胰腺癌组织及癌旁正常组织中miR-939-5p,ARHGAP4 mRNA的相对表达量,并分析二者表达与胰腺癌患者临床病理特征的关系,用Pearson线性相关法分析miR-939-5p,ARHGAP4 mRNA的关系....
The expression levels of circ_0058123, microRNA-939-5p (miR-939-5p) and Rac family small GTPase 1 (RAC1) were measured by quantitative real-time polymerase chain reaction or western blot assay. 5-Ethynyl-2'-deoxyuridine (EdU) incorporation assay, transwell assay, tube formation assay and ...
However, miR‐939‐5p did not show a regulatory effect on the 3′‐untranslated region of CD2AP. The expression levels of specific protein 1 and adenovirus E2 promoter‐binding factor 1, important transcription regulators in the promoter region of CD2AP, were also not affected by microRNA (miR...
Inflammation Related lncNOS2P3-miR-939-5p-iNOS/TNFα Pathway Inhibits Myocardial and Endothelial Cells Apoptosis in Chronic Heart Failuredoi:10.2139/ssrn.3444386LncRNA-NOS2P3MicroRNA-939-5pINOSTNFαApoptosisChronic heart failureBackground: Inflammatory cytokines induced cells apoptosis is important for ...
Interplay between the Pan-Tumor Suppressor miR-939-5p and the oncogenic lncRNA-HEIH dually curbs Hydrogen Sulphide and Nitric Oxide production in breast cancer cellsdoi:10.1016/S0959-8049(20)30714-0H. NafeaR. YounessK. Abou-AishaM. Gad...