本研究通过评估CRC患者血浆外泌体中miR-590-3p的表达水平及其与患者临床病理特征及预后的关系,证实了miR-590-3p在CRC患者血浆外泌体中呈高表达,且其高表达与多种不良临床病理因素及OS缩短相关;在调整其他混杂因素后,循环血浆外泌体miR-590-3p高...
实验结果显示,在高糖培养条件下,心肌成纤维细胞中miR-590-3p的表达水平显著升高。 2. miR-590-3p对高糖培养下心肌成纤维细胞焦亡的影响 通过RT-qPCR和Western blot检测发现,过表达miR-590-3p可促进高糖培养下心肌成纤维细胞的焦亡,而抑制miR-590-3p的表达则可抑制细胞的焦亡。同时,流式细胞术和免疫荧光染色结果也...
miR-590-3pMPTPMPP帕金森病凋亡自噬[目的]帕金森病(Parkinson's disease,PD)是临床上第二常见的神经退行性疾病,典型的病理改变是大脑黑质致密部(substantia nigra pars compacta,SNpc)多巴胺能神经元(Dopaminergic neuron,DAN)的丢失.现有研究证明,微小RNA(microRNA,miRNA)在PD的发生发展中扮演着重要角色,可以调节PD...
㊀㊀摘要:目的㊀观察微小ＲＮＡ￣５９０￣３ｐ(ｍｉＲ￣５９０￣３ｐ)对结直肠癌(ＣＲＣ)细胞顺铂(ＤＤＰ)耐药的影响ꎬ并探讨其可能的机制ꎮ方法㊀在ＣＲＣ细胞系ＳＷ４８０㊁ＳＷ６２０㊁ＨＣＴ１１６中选择ｍｉＲ￣５９０￣３ｐ表达最高的ＳＷ６２０细胞构建ＤＤＰ耐药株(ＤＤＰ/ＳＷ６２０)...
CAF-derived exosomes overexpressing miR-590-3p increased cell survival and the ratio of p-PI3K/PI3K and p-AKT/AKT while lowering the expressions of cleaved-PARP, cleaved-caspase 3 and 纬H2AX in cells. Furthermore, in vivo experimental results confirmed that CAF-derived exosomal miR-590-3p ...
In the present study, miR-590-3p, an anti-angiogenic miRNA, was predicted to target VEGFA, a key factor that can be transcriptionally upregulated by HIF1A during ADSC proliferation and tubule formation in response to hypoxic stimulation. Herein, we found that in response to hypoxic stimuli, ...
目的:探究miR-590-3p在甲状腺乳头状癌进展中的作用。方法:利用RT-PCR检测甲状腺乳头状癌患者标本及其细胞系中miR-590-3p的表达。利用CCK-8、EdU、划痕、Transwell实验以及细胞流式技术检测miR-590-3p对细胞相关功能的影响。Western blot检测上皮细胞-间充质转化(epithelial-mesenchymal transiti...
目的 应用无血清培养基分离胰腺癌干细胞,检测其miR-590-3p的表达.方法 运用无血清培养基克隆培养ASPC-1,PANC1细胞,检测其单克隆形成,分化及细胞周期,半数抑制浓度(IC50)和表面标记物CD24+, CD44+表达.实时定量PCR法检测细胞miR-590-3p的表达.结果 经无血清培养基培养,(0.94±0.53)%的ASPC-1细胞和(0.57±...
Furthermore, bioinformatics analysis demonstrated that SOX9 was a potential target of miR-590-3p. SOX9 was up-regulated in osteosarcoma tissues. Transfection with miR-590-3p mimics markedly suppressed SOX9 expression both at the mRNA level and protein level. Dual luciferase assay validated the direct...
The main aim of the study is to assess the levels of hsa-miR-590-3p and the role of its downstream target genes in HCC.2. Material and Methods 2.1. In Silico Analysis Several bioinformatics tools were used to predict the potential downstream target genes of hsa-miR-590-3p. For the ...