Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI. CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells[J].Journal of Zhejiang University Science B, 2022, 23(9): 732-...
(CASP8)protein activity in vitro in HO-induced K-562 cells.The circRNA.0007127-miR-513a-5p and CASP8-miR-513a-5p interactions were verified by luciferase reporter assays.Results:Silencing circRNA.0007127 decreased cell apoptosis by inhibiting CASP8 pathway activation in K-562 cells.Compared with...
circ_0001785 induce proliferation of endothelial cells and inhibit apoptosis and migration of endothelial cells. A Expression of circ_0001785 after co-culture with endothelial cells. B Analysis of cell viability by CCK-8 assay C Analysis of cell proliferation by EdU assay D Analysis of apoptosis by...
In contrast, neither the apoptosis inhibitor (VZAD), necrosis inhibitor (NEC-1), autophagy inhibitor (3-MA), nor pyroptosis inhibitor (BELNA) affected cell viability. (F-H) Knockdown of circTTC13 enhances ROS levels (F), reduces mitochondrial membrane potential (G), and increases Fe2+ ...
Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI. CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells[J]. Journal of Zhejiang University Science B , 2022, 23(9...
Simultaneous overexpression of miR-513a-5p and RANBP2 in GHINK- 1 cells significantly reduced the apoptosis rate (9.96±0. 72 vs I 5.94± 1.00, P<0.05). Conclusions The long-chain non-coding RNA (lncRNA) DLEU1 can promote the proliferation ...
APOPTOSISINHIBITORPROGNOSISCervical cancer is one of the most frequent types of cancer in women, which is characterized by high invasion and metastatic tendency in its advanced stage. Emerging evidence indicated that long non-coding RNAs (LncRNAs) are involved in the pathogenesis of cervical cancer. ...
Flow cytometry was performed to assess the apoptosis. Oxidative stress, inflammation and endothelial dysfunction were evaluated using enzyme-linked immunosorbent assay (ELISA) and western blotting. The levels of miR-513a-5p were assessed using quantitative real-time polymerase chain ...
Results: The vitro co-cultured results showed that the amount of plasma-derived exosomes have an increase in patients with coronary artery disease, and the inflammation and apoptosis of endothelial cells were exacerbated. Over-expression of circ_0001785 reduced endothelial cell injury through the ceRNA...